Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling

ČERNÝ, Martin, Filip DYČKA, Janette BOBÁĽOVÁ and Břetislav BRZOBOHATÝ. Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling. In FEBS Journal. Roč. 276. 2009. ISSN 1742-464X.

Basic information

• Original name: Early cytokinin response proteins and phosphoproteins of Arabidopsis thaliana identified by proteome and phosphoproteome profiling
• Name in Czech: Změny v proteomu a fosfoproteomu v brzké odpovědi na cytokininy v Arabidopsis thaliana
• Authors: ČERNÝ, Martin, Filip DYČKA, Janette BOBÁĽOVÁ and Břetislav BRZOBOHATÝ.
• Edition: FEBS Journal. Roč. 276, 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Impact factor: Impact factor: 3.042
• Organization unit: Faculty of Science
• ISSN: 1742-464X
• UT WoS: 000267069900685
• Keywords in English: proteomic analysis; phosphoproteome; cytokinin
• Tags: Arabidopsis, cytokinins, MALDI, mass spectrometry, TOF
• Tags: International impact

Abstract

We employed 2-DE followed by image analysis and MALDI-TOF-TOF MS to analyze early changes in steady-state protein levels and phosphorylation status of the proteome in cytokinin treated Arabidopsis seedlings. Effects of four principal cytokinins (trans-zeatin, benzylaminopurine, thidiazuron and 6-(Dimethylallylamino)purine) were compared.

Abstract (in Czech)

V této práci byly sledovány změny proteinového a fosfoproteinového profilu rostliny Arabidopsis v odpovědi na cytokininy pomocí metod 2-DE, analýzou obrazu a MALDI-TOF-TOF MS.

Links

• IAA600040701, research and development project: Name: Dynamika proteomu v odpovědi na zvýšené hladiny cytokininů u Arabidopsis

Investor: Academy of Sciences of the Czech Republic, Proteome dynamics in response to increased cytokinin levels in Arabidopsis

• 1M06030, research and development project: Name: Funkční genomika a proteomika ve šlechtění rostlin