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@proceedings{854409,
author = {Dubový, Petr and David, Marek and Hradilová Svíženská, Ivana and Klusáková, Ilona},
booktitle = {9th European Meeting on Glial Cells in Health and Disease},
keywords = {behavioral tests; bilateral roots reaction; dorsal root; endoneurium; laminin-1; neuropathic pain; peripheral nervous system; spinal nerve roots; unilateral constriction of sciatic nerve; ventral root},
language = {eng},
title = {Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array},
url = {http://www.glialcells2009paris.com},
year = {2009}
}
TY - CONF
ID - 854409
AU - Dubový, Petr - David, Marek - Hradilová Svíženská, Ivana - Klusáková, Ilona
PY - 2009
TI - Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array
KW - behavioral tests
KW - bilateral roots reaction
KW - dorsal root
KW - endoneurium
KW - laminin-1
KW - neuropathic pain
KW - peripheral nervous system
KW - spinal nerve roots
KW - unilateral constriction of sciatic nerve
KW - ventral root
UR - http://www.glialcells2009paris.com
N2 - Bilateral changes of immunofluorescence for laminin-1 (lam-1) were investigated in the rat spinal nerve roots (L4-L5) following neuropathic pain induction by unilateral sciatic nerve ligature. Induction of neuropathic pain was confirmed in experimental animals by behavioral tests of mechanical allodynia and thermal hyperalgesia. Dorsal and ventral roots of naive rats displayed lam-1 immunostaining in the basal laminae on outer surface of Schwann cell/axon units, perineurial sheaths and blood vessels. Significantly higher brightness of immunofluorescence for lam-1 was observed in the ventral than dorsal roots removed from naive rats as well as those operated on sciatic nerve ligature and surviving for 1 or 3 days. Chronic constriction injury of sciatic nerve for 7 and 14 days results in reduction of different levels of immunofluorescence brightness between dorsal and ventral roots. In comparison to naive and sham-operated animals, apparent bilateral decrease of immunofluorescence brightness for lam-1 was found in both dorsal and ventral roots 7 days after unilateral nerve injury. Although the pattern of lam-1 alterations in both ipsi- and contralateral roots was similar, changes of immunofluorescence were more evident in ipsilateral than contralateral roots. The results indicate that quantitative changes of immunofluorescence for lam-1 are present bilaterally in proximal segments of peripheral nerve far from unilateral chronic constriction injury inducing neuropathic pain. This suggests a possible mechanism for misbalance alongside the afferent and motor axons, the involvement of which in neuropathic pain induction is assumed.
ER -
DUBOVÝ, Petr, Marek DAVID, Ivana HRADILOVÁ SVÍŽENSKÁ a Ilona KLUSÁKOVÁ. Bilateral quantitative changes of laminin-1 in the dorsal and ventral roots following unilateral chronic constriction injury of sciatic nerve - An immunofluorescence tissue array. Online. In \textit{9th European Meeting on Glial Cells in Health and Disease}. 2009. ISSN~0894-1491. [citováno 2024-04-24]
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