MATULA, Petr, Fatima VERISSIMO, Stefan WÖRZ, Roland EILS, Rainer PEPPERKOK and Karl ROHR. Quantification of Fluorescent Spots in Time Series of 3D Confocal Microscopy Images of Endoplasmic Reticulum Exit Sites Based on the HMAX Transform. In Proceedings of SPIE Medical Imaging. Neuveden: Neuveden, 2010. 7 pp. ISBN 978-0-8194-8027-9.
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Basic information
Original name Quantification of Fluorescent Spots in Time Series of 3D Confocal Microscopy Images of Endoplasmic Reticulum Exit Sites Based on the HMAX Transform
Authors MATULA, Petr (203 Czech Republic, guarantor, belonging to the institution), Fatima VERISSIMO (620 Portugal), Stefan WÖRZ (276 Germany), Roland EILS (276 Germany), Rainer PEPPERKOK (276 Germany) and Karl ROHR (276 Germany).
Edition Neuveden, Proceedings of SPIE Medical Imaging, 7 pp. 2010.
Publisher Neuveden
Other information
Original language English
Type of outcome Proceedings paper
Field of Study 10201 Computer sciences, information science, bioinformatics
Country of publisher United States of America
Confidentiality degree is not subject to a state or trade secret
WWW URL
RIV identification code RIV/00216224:14330/10:00042889
Organization unit Faculty of Informatics
ISBN 978-0-8194-8027-9
UT WoS 000284752800047
Keywords in English Fluorescence microscopy; spot detection; HMAX transform; endoplasmic reticulum exit sites
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Petr Matula, Ph.D., učo 3019. Changed: 14/1/2011 15:37.
Abstract
We present an approach for the quantification of fluorescent spots in time series of 3-D confocal microscopy images of endoplasmic reticulum exit sites of dividing cells. Fluorescent spots are detected based on extracted image regions of highest response using the HMAX transform and prior convolution of the 3-D images with a Gaussian kernel. The sensitivity of the involved parameters was studied and a quantitative evaluation using both 3-D synthetic and 3-D real data was performed. The approach was successfully applied to more than one thousand 3-D confocal microscopy images.
Links
MSM0021622419, plan (intention)Name: Vysoce paralelní a distribuované výpočetní systémy
Investor: Ministry of Education, Youth and Sports of the CR, Research Intents
2B06052, research and development projectName: Vytipování markerů, screening a časná diagnostika nádorových onemocnění pomocí vysoce automatizovaného zpracování multidimenzionálních biomedicínských obrazů (Acronym: Biomarker)
Investor: Ministry of Education, Youth and Sports of the CR, Health and quality of life
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