Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening

HÉGROVÁ, Blanka, Marta FARKOVÁ, Simona MACUCHOVÁ, Josef HAVEL and Jan PREISLER. Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening. Journal of Separation Science. WILEY-VCH Verlag GmbH & Co., 2009, vol. 32, No 22, p. 1-7. ISSN 1615-9306.

Basic information

Original name

Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening

Name in Czech

Studium vztahu mezi stresovými peptidy ječmene a přepěnováním piva pomocí SDS-PAGE a MS

Authors

HÉGROVÁ, Blanka (203 Czech Republic), Marta FARKOVÁ (203 Czech Republic, belonging to the institution), Simona MACUCHOVÁ (203 Czech Republic), Josef HAVEL (203 Czech Republic, belonging to the institution) and Jan PREISLER (203 Czech Republic, guarantor, belonging to the institution)

Edition

Journal of Separation Science, WILEY-VCH Verlag GmbH & Co. 2009, 1615-9306

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10406 Analytical chemistry

Country of publisher

Germany

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.551

RIV identification code

RIV/00216224:14310/09:00029652

Organization unit

Faculty of Science

UT WoS

000274217600032

Keywords in English

Antifungal peptides; Barley; Gushing; MALDI-TOF MS; SDS-PAGE

Tags

International impact, Reviewed

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Abstract

V originále

The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (|r|Ao0.07, 0.344), (|r|Ao0.01, 0.494), respectively, as detected by both methods.

In Czech

Anotace článku Studium vztahu mezi stresovými peptidy ječmene a přepěnováním piva pomocí SDS-PAGE a MS - viz anglický text.

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Links

GA525/06/0663, research and development project	Name: Sledování chemických změn obilky ječmene po napadení patogeny pro kontrolu a zvýšení kvality ječmene a následných potravinářských produktů Investor: Czech Science Foundation, Study of the chemical changes of barley caryopses after an attack by pathogens to monitor and improve the quality of barley and its derived food products
LC06035, research and development project	Name: Centrum biofyzikální chemie, bioelektrochemie a bioanalýzy. Nové nástroje pro genomiku, proteomiku a biomedicínu. Investor: Ministry of Education, Youth and Sports of the CR, Centre of Biophysical Chemistry, Bioelectrochemistry and Bioanalysis. New Tools for Genomics, Proteomics and Biomedicine
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations