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@inproceedings{858664,
author = {Ďurech, Michal and Adam, Jan and Kříž, Zdeněk and Kostlánová, Nikola and Koča, Jaroslav and Wimmerová, Michaela},
address = {Brno},
booktitle = {XIII. Setkání biochemiků a molekulárních biologů},
keywords = {lectin engineering; molecular modeling; thermodynamics},
language = {eng},
location = {Brno},
isbn = {978-80-210-4830-0},
pages = {109-109},
publisher = {Masarykova Univerzita},
title = {Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study},
year = {2009}
}
TY - JOUR
ID - 858664
AU - Ďurech, Michal - Adam, Jan - Kříž, Zdeněk - Kostlánová, Nikola - Koča, Jaroslav - Wimmerová, Michaela
PY - 2009
TI - Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study
PB - Masarykova Univerzita
CY - Brno
SN - 9788021048300
KW - lectin engineering
KW - molecular modeling
KW - thermodynamics
N2 - Our research is focused on bacterial lectin RS20L from Ralstonia solanacearum.The RS20L lectin displays a different structure from these two, and no structure homologue was found for this lectin in the bacterial lectin realm. Its fold resembles animal realm galectins. The native lectin structure was crystallized, and during the experimental preparation of a recombinant form and attempts of functional characterization, several cases of very peculiar behavior were recorded extremely high stability of trimeric form, irreversible loss of binding properties after purification, etc. The research focuses on investigating the general and binding properties of the RS20L lectin that could help to explain this unusual behaviour. The study was performed by means of computational chemistry and molecular modelling. Several promising hints were found that could, upon further investigation, help to decrypt the reasons behind the lectin behavior.
ER -
ĎURECH, Michal, Jan ADAM, Zdeněk KŘÍŽ, Nikola KOSTLÁNOVÁ, Jaroslav KOČA and Michaela WIMMEROVÁ. Revealing the mysteries of the RS20L lectin from Ralstonia solanacearum ; a molecular modelling study. In \textit{XIII. Setkání biochemiků a molekulárních biologů}. Brno: Masarykova Univerzita, 2009, p.~109. ISBN~978-80-210-4830-0.
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