| SIERRA, Colavito, Macris-Kiss MARGARET, Seong CHANGHYUN, Gleeson OLIVE, Greene ERIC C., Klein HANNAH L., Krejci LUMIR and Sung PATRICK. Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. Nucleic Acids Research. ENGLAND: OXFORD UNIV PRESS, 2009, vol. 20, No 37, p. 6754-64, 10 pp. ISSN 0305-1048. |
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@article{860038,
author = {Sierra, Colavito and Margaret, MacrisandKiss and Changhyun, Seong and Olive, Gleeson and Eric C., Greene and Hannah L., Klein and Lumir, Krejci and Patrick, Sung},
article_location = {ENGLAND},
article_number = {37},
keywords = {DNA repair; DNA damage; replication; genomic instability},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption.},
url = {http://www.ncbi.nlm.nih.gov/pubmed/19745052?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1},
volume = {20},
year = {2009}
}
TY - JOUR
ID - 860038
AU - Sierra, Colavito - Margaret, Macris-Kiss - Changhyun, Seong - Olive, Gleeson - Eric C., Greene - Hannah L., Klein - Lumir, Krejci - Patrick, Sung
PY - 2009
TI - Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption.
JF - Nucleic Acids Research
VL - 20
IS - 37
SP - 6754-64
EP - 6754-64
PB - OXFORD UNIV PRESS
SN - 03051048
KW - DNA repair
KW - DNA damage
KW - replication
KW - genomic instability
UR - http://www.ncbi.nlm.nih.gov/pubmed/19745052?itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum&ordinalpos=1
N2 - The SRS2 (Suppressor of RAD Six screen mutant 2) gene encodes an ATP-dependent DNA helicase that regulates homologous recombination in Saccharomyces cerevisiae. Mutations in SRS2 result in a hyper-recombination phenotype, sensitivity to DNA damaging agents and synthetic lethality with mutations that affect DNA metabolism. Several of these phenotypes can be suppressed by inactivating genes of the RAD52 epistasis group that promote homologous recombination, implicating inappropriate recombination as the underlying cause of the mutant phenotype. Consistent with the genetic data, purified Srs2 strongly inhibits Rad51-mediated recombination reactions by disrupting the Rad51-ssDNA presynaptic filament. Srs2 interacts with Rad51 in the yeast two-hybrid assay and also in vitro. To investigate the functional relevance of the Srs2-Rad51 complex, we have generated srs2 truncation mutants that retain full ATPase and helicase activities, but differ in their ability to interact with Rad51. Importantly, the srs2 mutant proteins attenuated for Rad51 interaction are much less capable of Rad51 presynaptic filament disruption. An internal deletion in Srs2 likewise diminishes Rad51 interaction and anti-recombinase activity. We also present evidence that deleting the Srs2 C-terminus engenders a hyper-recombination phenotype. These results highlight the importance of Rad51 interaction in the anti-recombinase function of Srs2, and provide evidence that this Srs2 function can be uncoupled from its helicase activity.
ER -
SIERRA, Colavito, Macris-Kiss MARGARET, Seong CHANGHYUN, Gleeson OLIVE, Greene ERIC C., Klein HANNAH L., Krejci LUMIR and Sung PATRICK. Functional significance of the Rad51-Srs2 complex in Rad51 presynaptic filament disruption. \textit{Nucleic Acids Research}. ENGLAND: OXFORD UNIV PRESS, 2009, vol.~20, No~37, p.~6754-64, 10 pp. ISSN~0305-1048.
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