Avoidance tests of solid waste samples with Enchytraeus albidus, Enchytraeus crypticus and Eisenia fetida

KOBETIČOVÁ, Klára and Jakub HOFMAN. Avoidance tests of solid waste samples with Enchytraeus albidus, Enchytraeus crypticus and Eisenia fetida. Online. In SETAC Europe 19th Annual Meeting, from 31 May until 4 June 2009, Göteborg, Sweden. 2009, [citováno 2024-04-24]

Basic information

• Original name: Avoidance tests of solid waste samples with Enchytraeus albidus, Enchytraeus crypticus and Eisenia fetida
• Name in Czech: Testy únikového chování s druhy Enchytraeus albidus, Enchytraeus crypticus a Eisenia fetida na pevných vzorcích odpadů
• Name (in English): Avoidance tests of solid waste samples with Enchytraeus albidus, Enchytraeus crypticus and Eisenia fetida
• Authors: KOBETIČOVÁ, Klára and Jakub HOFMAN
• Edition: SETAC Europe 19th Annual Meeting, from 31 May until 4 June 2009, Göteborg, Sweden, 2009.

Other information

• Original language: Czech
• Type of outcome: Conference abstract
• Field of Study: 10511 Environmental sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords (in Czech): test únikového chování, ekotoxicity odpadů, roupice, žížala
• Keywords in English: avoidance test, waste ecotoxicity, enchytraeid, earthworm

Abstract

Contact bioassays are important for testing ecotoxicity of solid materials. However, survival and reproduction tests are often not practical due to their duration (up to several weeks). Moreover, mortality as an endpoint is robust parameter not useful as an early warning. From these reasons, the avoidance tests with soil invertebrates suggest good alternative or extension to the classic test batteries. They offer very short time duration (days) and sensitive subacute endpoint (behavior). The aim of our study was to compare sensitivity of three oligochaete species (enchytraeids Enchytraeus albidus, Enchytraeus crypticus and earthworm Eisenia fetida) in avoidance tests of three types of wastes. Incineration ash, contaminated soil and contaminated wood chips were diluted with artificial soil in scaled ratios and clear artificial soil was used as control. The avoidance tests lasted 2 days and they were designed as two-chamber test systems. The toxicity of wastes decreased in this order: wood > ash > soil for all used species. E. fetida was the most sensitive species and E. crypticus the least sensitive one (E. fetida: EC50ash < 3.13%, EC50wood < 4.5%, EC50soil < 25%; E. albidus: EC50ash = 4.42%, EC50wood = 9.55%, EC50soil > 100%; E. crypticus: EC50ash = 14.33%, EC50wood = 9.17%, EC50soil > 100%). Our results indicate that avoidance tests with earthworms and enchytraeids are feasible for waste testing

Abstract (in English)

Contact bioassays are important for testing ecotoxicity of solid materials. However, survival and reproduction tests are often not practical due to their duration (up to several weeks). Moreover, mortality as an endpoint is robust parameter not useful as an early warning. From these reasons, the avoidance tests with soil invertebrates suggest good alternative or extension to the classic test batteries. They offer very short time duration (days) and sensitive subacute endpoint (behavior). The aim of our study was to compare sensitivity of three oligochaete species (enchytraeids Enchytraeus albidus, Enchytraeus crypticus and earthworm Eisenia fetida) in avoidance tests of three types of wastes. Incineration ash, contaminated soil and contaminated wood chips were diluted with artificial soil in scaled ratios and clear artificial soil was used as control. The avoidance tests lasted 2 days and they were designed as two-chamber test systems. The toxicity of wastes decreased in this order: wood > ash > soil for all used species. E. fetida was the most sensitive species and E. crypticus the least sensitive one (E. fetida: EC50ash < 3.13%, EC50wood < 4.5%, EC50soil < 25%; E. albidus: EC50ash = 4.42%, EC50wood = 9.55%, EC50soil > 100%; E. crypticus: EC50ash = 14.33%, EC50wood = 9.17%, EC50soil > 100%). Our results indicate that avoidance tests with earthworms and enchytraeids are feasible for waste testing

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)