Avoidance test with Enchytraeus albidus: Is avoidance of individual worms comparable to avoidance of grouped worms?

KOBETIČOVÁ, Klára, Jakub HOFMAN and Ivan HOLOUBEK. Avoidance test with Enchytraeus albidus: Is avoidance of individual worms comparable to avoidance of grouped worms? In SETAC Europe 19th Annual Meeting, from 31 May until 4 June 2009, Göteborg, Sweden. 2009.

Basic information

• Original name: Avoidance test with Enchytraeus albidus: Is avoidance of individual worms comparable to avoidance of grouped worms?
• Name in Czech: Test únikového chování s druhem Enchytraeus albidus: je únik jednotlivých červů srovnatelný s únikou reakcí červů ve skupinách?
• Authors: KOBETIČOVÁ, Klára, Jakub HOFMAN and Ivan HOLOUBEK.
• Edition: SETAC Europe 19th Annual Meeting, from 31 May until 4 June 2009, Göteborg, Sweden, 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: 10600 1.6 Biological sciences
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Science
• Keywords (in Czech): test únikového chování, karbendazim, design testu, roupice
• Keywords in English: avoidance test, carbendazim, test design, enchytraeid

Abstract

The avoidance tests are based on a presumption that each individual organism which is introduced into the test vessel behaves independently of the other individuals. This is usually verified by using dual-controls with clear substrate on the both sides of the test vessel when the same number of individuals is expected to be on the both sides at the end of the test. In our study, the linkage between individual worm and the whole group was verified on the species Enchytraeus albidus. One or ten individuals were introduced into the test vessels containing clean LUFA 2.2 soil on the one side and the same soil contaminated by pesticide carbendazim on the other side. Concentration used was derived from EC50 value (7.6 mg/kg) of preliminary avoidance test. The reaction of worms was measured after 3, 6, 12, 24 and 48 hours. The results indicated that worms in groups needed 12 – 24 hours to show significant avoidance response while single worms started to significantly prefer control soil after longer time (48 hours). Nevertheless, it seems that after sufficient time (> 2 days) the overall result of the test run in both designs showed similar results. Our results demonstrated that worms in groups move independently and their avoidance behavior does not really differ significantly from the behavior of individual worms.

Links

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)