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@article{865900,
author = {Hežová, Renata and Slabý, Ondřej and Faltejsková, Petra and Mikulková, Zuzana and Burešová, Ivana and Muthu Raja, Karthick Raja and Hodek, Jan and Ovesná, Jaroslava and Michálek, Jaroslav},
article_number = {260},
keywords = {microRNA; Expression profile; Regulatory T cells; Diabetes mellitus type 1},
language = {eng},
issn = {0008-8749},
journal = {Cellular Immunology},
title = {microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients},
url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCF-4XJ17PW-4&_user=835458&_coverDate=12%2F31%2F2010&_rdoc=4&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236737%232010%23997399997%231577274%23FLA%23display%23Volume)&_cdi=6737&_sort=d&_docancho},
volume = {2010},
year = {2010}
}
TY - JOUR
ID - 865900
AU - Hežová, Renata - Slabý, Ondřej - Faltejsková, Petra - Mikulková, Zuzana - Burešová, Ivana - Muthu Raja, Karthick Raja - Hodek, Jan - Ovesná, Jaroslava - Michálek, Jaroslav
PY - 2010
TI - microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients
JF - Cellular Immunology
VL - 2010
IS - 260
SP - 70-74
EP - 70-74
PB - Elsevier
SN - 00088749
KW - microRNA
KW - Expression profile
KW - Regulatory T cells
KW - Diabetes mellitus type 1
UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WCF-4XJ17PW-4&_user=835458&_coverDate=12%2F31%2F2010&_rdoc=4&_fmt=high&_orig=browse&_srch=doc-info(%23toc%236737%232010%23997399997%231577274%23FLA%23display%23Volume)&_cdi=6737&_sort=d&_docancho
N2 - We performed miRNAs profiling in a population of regulatory T celss (Tregs) separated from peripheral blood of five type 1 diabetic patients and six healthy donors. For more detailed molecular char- acterisation of Tregs, we additionally compared miRNAs expression profiles of Tregs and conventional T cells. Tregs were isolated according to CD3+, CD4+, CD25hi+ and CD127low by flow cytometry, and miRNA expression profiling was performed using TaqMan Array Human MicroRNA Panel-1 (384-well low density array). In Tregs of diabetic patients we found significantly increased expression of miRNA-510 (p = 0.05) and decreased expression of both miRNA-342 (p< 0.0001) and miRNA-191 (p = 0.0079). When comparing Tregs and T cells, we revealed that Tregs had significant higher expression of miRNA-146a and lower expression of eight specific miRNAs (20b, 31, 99a, 100, 125b, 151, 335, and 365). This is the first study demonstrating changes in miRNA expression profiles occurring in Tregs of T1D patients and a miRNAs signature of adult Tregs.
ER -
HEŽOVÁ, Renata, Ondřej SLABÝ, Petra FALTEJSKOVÁ, Zuzana MIKULKOVÁ, Ivana BUREŠOVÁ, Karthick Raja MUTHU RAJA, Jan HODEK, Jaroslava OVESNÁ and Jaroslav MICHÁLEK. microRNA-342, microRNA-191 and microRNA-510 are differentially expressed in T regulatory cells of type 1 diabetic patients. \textit{Cellular Immunology}. Elsevier, 2010, vol.~2010, No~260, p.~70-74. ISSN~0008-8749.
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