| JUŘICA, Jan, Michal KÝR, Eva MCCASKEY HADAŠOVÁ and Josef TOMANDL. Evaluation of the activity of P450 enzymes in rats: use of the single marker or combined drug administration. Neuroendocrinology Letters. 2009, vol. 2009, No 30, p. 92-95. ISSN 0172-780X. |
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@article{866031,
author = {Juřica, Jan and Kýr, Michal and McCaskey Hadašová, Eva and Tomandl, Josef},
article_number = {30},
keywords = {P450; rat; metabolic activity; evaluation; marker combination},
language = {eng},
issn = {0172-780X},
journal = {Neuroendocrinology Letters},
title = {Evaluation of the activity of P450 enzymes in rats: use of the single marker or combined drug administration.},
volume = {2009},
year = {2009}
}
TY - JOUR
ID - 866031
AU - Juřica, Jan - Kýr, Michal - McCaskey Hadašová, Eva - Tomandl, Josef
PY - 2009
TI - Evaluation of the activity of P450 enzymes in rats: use of the single marker or combined drug administration.
JF - Neuroendocrinology Letters
VL - 2009
IS - 30
SP - 92-95
EP - 92-95
SN - 0172780X
KW - P450
KW - rat
KW - metabolic activity
KW - evaluation
KW - marker combination
N2 - A "cocktail" of several substrates is frequently used to assess metabolic activity of multiple cytochrome P450 enzymes in one session. Some interactions among substrates can appear and may influence the rate of biotransformation of other ones. Our current work was aimed on the influence of tolbutamide on cytochrome P450-mediated metabolism of phenacetin and vice versa. In the presented work, the biotransformation rates of phenacetin and tolbutamide (markers of rat CYP1A2 and CYP2C6/11 metabolic activities, respectively) administered either separately or both simultaneously were compared. The model of isolated perfused rat liver was used. Phenacetin had no significant effect on tolbutamide hydroxylation. Tolbutamide addition to the perfusion medium significantly increased the rate of O-deethylation of phenacetin. Some differences in the rate of P450-mediated metabolism can be observed when comparing assessment using combination of two model substrates with the common way (single marker administration). Due to these differences, results obtained by the mentioned methodologies might not be fully comparable.
ER -
JUŘICA, Jan, Michal KÝR, Eva MCCASKEY HADAŠOVÁ and Josef TOMANDL. Evaluation of the activity of P450 enzymes in rats: use of the single marker or combined drug administration. \textit{Neuroendocrinology Letters}. 2009, vol.~2009, No~30, p.~92-95. ISSN~0172-780X.
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