| MASAŘÍK, Michal, Dalibor HÚSKA, Vojtěch ADAM, V. Jaroslav BURDA, Tomáš ECKSCHLAGER, Marie STIBOROVÁ a René KIZEK. Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions. International Journal of Molecular Medicine. 2009, roč. 24, Suppl. 1, s. S49, 1 s. ISSN 1107-3756. |
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@article{868047,
author = {Masařík, Michal and Húska, Dalibor and Adam, Vojtěch and Burda, V. Jaroslav and Eckschlager, Tomáš and Stiborová, Marie and Kizek, René},
article_number = {Suppl. 1},
keywords = {Doxorubicin; DNA; electrochemistry; drug-DNA interaction},
language = {eng},
issn = {1107-3756},
journal = {International Journal of Molecular Medicine},
title = {Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions},
volume = {24},
year = {2009}
}
TY - JOUR
ID - 868047
AU - Masařík, Michal - Húska, Dalibor - Adam, Vojtěch - Burda, V. Jaroslav - Eckschlager, Tomáš - Stiborová, Marie - Kizek, René
PY - 2009
TI - Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions
JF - International Journal of Molecular Medicine
VL - 24
IS - Suppl. 1
SP - S49
EP - S49
SN - 11073756
KW - Doxorubicin
KW - DNA
KW - electrochemistry
KW - drug-DNA interaction
N2 - Anthracyclines including doxorubicin are commonly used in the treatment of a number of diverse malignant. There is compelling evidence that cellular DNA is the primary target for anthracyclines. In this study we aimed at investigation of intercalation of doxorubicin into DNA in vitro by using of square wave voltammetry coupled with adsorptive transfer stripping technique. Based on the decline of the signal of adenine and cytosine, we indicated binding of doxorubicin into dsDNA. The electrochemical method was further utilized for detection of doxorubicin-DNA adducts in neuroblastoma cells. Prior to electrochemical analysis dsDNA was carefully isolated from these cells to avoid modification of DNA bases. We observed significant (a=0.05) difference in DNA signals after intercalation of doxorubicin between resistant and sensitive cells. For more comprehensive understanding of the intercalation, computational experiments using design for testability approach with correction on dispersion interaction were performed. Several molecular structures were explored and clear preference for very stable stacked structures was observed.
ER -
MASAŘÍK, Michal, Dalibor HÚSKA, Vojtěch ADAM, V. Jaroslav BURDA, Tomáš ECKSCHLAGER, Marie STIBOROVÁ a René KIZEK. Doxorubicin and genomic DNA, new insights in drug-nucleic acids interactions. \textit{International Journal of Molecular Medicine}. 2009, roč.~24, Suppl. 1, s.~S49, 1 s. ISSN~1107-3756.
|
