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@article{869286,
author = {Bernard, Vladan and Škorpíková, Jiřina and Mornstein, Vojtěch and Slaninová, Iva},
article_number = {3},
keywords = {Carcinoma cells; Cisplatin; Ultrasound; Viability; MTT test},
language = {eng},
issn = {0041-624X},
journal = {Ultrasonics},
title = {Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells},
volume = {50},
year = {2010}
}
TY - JOUR
ID - 869286
AU - Bernard, Vladan - Škorpíková, Jiřina - Mornstein, Vojtěch - Slaninová, Iva
PY - 2010
TI - Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells
JF - Ultrasonics
VL - 50
IS - 3
SN - 0041624X
KW - Carcinoma cells
KW - Cisplatin
KW - Ultrasound
KW - Viability
KW - MTT test
N2 - Abstract- The effects of low-power ultrasound, the anti-cancer drug cisplatin, and their combined application were studied in two lines of human ovarian carcinoma cells, A2780 and A2780cis. Four modes of treatment were used: exposure to ultrasonic field, application of cisplatin, exposure to ultrasound followed by cisplatin, and presence of cisplatin followed by exposure to application ultrasound. Ultrasound was used at intensities of 0.5 W/cm(2) and 1.0 W/cm(2) for 10 min, cisplatin was applied at concentrations of 1 mu M and 6 mu M per cell suspension treated in A2780 and cisplatin-resistant A2780cis cells, respectively. The results of each experimental treatment were assessed by the resultant cell viability related to the viability of control cells, using a standard MTT test. It was shown that a combined effect of ultrasound and cisplatin was more effective than that of ultrasound or cisplatin alone. It also appeared that the order of application played a role, with the cisplatin-ultrasound treatment lowering cell viability more than the ultrasound-cisplatin treatment. It can be assumed that the exposure of cells to a low-power ultrasonic field has an immediate effect on the structure of cell surfaces and, consequently, on entry of cisplatin into the cell.
ER -
BERNARD, Vladan, Jiřina ŠKORPÍKOVÁ, Vojtěch MORNSTEIN and Iva SLANINOVÁ. Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells. \textit{Ultrasonics}. 2010, vol.~50, No~3, 357-362. ISSN~0041-624X.
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