J 2010

Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells

BERNARD, Vladan, Jiřina ŠKORPÍKOVÁ, Vojtěch MORNSTEIN and Iva SLANINOVÁ

Basic information

Original name

Biological effects of combined ultrasound and cisplatin treatment on ovarian carcinoma cells

Authors

BERNARD, Vladan (203 Czech Republic, guarantor, belonging to the institution), Jiřina ŠKORPÍKOVÁ (203 Czech Republic, belonging to the institution), Vojtěch MORNSTEIN (203 Czech Republic, belonging to the institution) and Iva SLANINOVÁ (203 Czech Republic, belonging to the institution)

Edition

Ultrasonics, 2010, 0041-624X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 1.599

RIV identification code

RIV/00216224:14110/10:00043266

Organization unit

Faculty of Medicine

UT WoS

000273954700004

Keywords in English

Carcinoma cells; Cisplatin; Ultrasound; Viability; MTT test
Změněno: 12/1/2011 12:31, doc. Mgr. Vladan Bernard, Ph.D.

Abstract

V originále

Abstract- The effects of low-power ultrasound, the anti-cancer drug cisplatin, and their combined application were studied in two lines of human ovarian carcinoma cells, A2780 and A2780cis. Four modes of treatment were used: exposure to ultrasonic field, application of cisplatin, exposure to ultrasound followed by cisplatin, and presence of cisplatin followed by exposure to application ultrasound. Ultrasound was used at intensities of 0.5 W/cm(2) and 1.0 W/cm(2) for 10 min, cisplatin was applied at concentrations of 1 mu M and 6 mu M per cell suspension treated in A2780 and cisplatin-resistant A2780cis cells, respectively. The results of each experimental treatment were assessed by the resultant cell viability related to the viability of control cells, using a standard MTT test. It was shown that a combined effect of ultrasound and cisplatin was more effective than that of ultrasound or cisplatin alone. It also appeared that the order of application played a role, with the cisplatin-ultrasound treatment lowering cell viability more than the ultrasound-cisplatin treatment. It can be assumed that the exposure of cells to a low-power ultrasonic field has an immediate effect on the structure of cell surfaces and, consequently, on entry of cisplatin into the cell.

Links

GA305/05/2030, research and development project
Name: Nové přístupy k chemoterapii rakoviny látkami odvozenými od sloučenin kovů
Investor: Czech Science Foundation
LC06077, research and development project
Name: Centrum chemické genetiky
Investor: Ministry of Education, Youth and Sports of the CR
MUNI/C/0092/2009, interní kód MU
Name: Proliferace a další parametry buněčných kultur po kombinované aplikaci ultrazvuku a vybraných cytostatik
Investor: Masaryk University, Category C - centralized resources + specific research