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@article{870093,
author = {Rudolf, Ivo and Mendel, Jan and Šikutová, Silvie and Švec, Pavel and Masaříková, Jana and Nováková, Dana and Buňková, Leona and Sedláček, Ivo and Hubálek, Zdeněk},
article_location = {Praha},
article_number = {5},
keywords = {16S rRNA; Identification; Ixodes ricinus; Dermacentor reticulatus; Haemaphysalis concinna; Ticks},
language = {eng},
issn = {0015-5632},
journal = {Folia Microbiologica},
title = {16S rRNA Gene-Based Identification of Cultured Bacterial Flora from Host-Seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna Ticks, Vectors of Vertebrate Pathogens},
volume = {54},
year = {2009}
}
TY - JOUR
ID - 870093
AU - Rudolf, Ivo - Mendel, Jan - Šikutová, Silvie - Švec, Pavel - Masaříková, Jana - Nováková, Dana - Buňková, Leona - Sedláček, Ivo - Hubálek, Zdeněk
PY - 2009
TI - 16S rRNA Gene-Based Identification of Cultured Bacterial Flora from Host-Seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna Ticks, Vectors of Vertebrate Pathogens
JF - Folia Microbiologica
VL - 54
IS - 5
SP - 419-428
EP - 419-428
PB - Springer
SN - 00155632
KW - 16S rRNA
KW - Identification
KW - Ixodes ricinus
KW - Dermacentor reticulatus
KW - Haemaphysalis concinna
KW - Ticks
N2 - A total of 151 bacterial isolates were recovered from different developmental stages of field-collected ticks. Microorganisms were identified by means of 16S rRNA gene sequencing. Almost 87 % of the strains belonged to G+ bacteria with predominantly occurring genera Bacillus and Paenibacillus. Other G+ strains included Arthrobacter, Corynebacterium, Frigoribacterium, Kocuria, Microbacterium, Micrococcus, Plantibacter, Rhodococcus, Rothia, and Staphylococcus. G-strains occurred less frequently, comprising genera Advenella, Pseudomonas, Rahnella, Stenotrophomonas, and Xanthomonas. Several strains of medical importance were found, namely Advenella incenata, Corynebacterium aurimucosum, Microbacterium oxydans, M. schleiferi, Staphylococcus spp., and Stenotrophomonas maltophilia. Data on cultivable microbial diversity in Eurasian tick species D. reticulatus and H. concinna are given, along with the extension of present knowledge concerning bacterial flora of I. ricinus.
ER -
RUDOLF, Ivo, Jan MENDEL, Silvie ŠIKUTOVÁ, Pavel ŠVEC, Jana MASAŘÍKOVÁ, Dana NOVÁKOVÁ, Leona BUŇKOVÁ, Ivo SEDLÁČEK and Zdeněk HUBÁLEK. 16S rRNA Gene-Based Identification of Cultured Bacterial Flora from Host-Seeking Ixodes ricinus, Dermacentor reticulatus and Haemaphysalis concinna Ticks, Vectors of Vertebrate Pathogens. \textit{Folia Microbiologica}. Praha: Springer, 2009, vol.~54, No~5, p.~419-428. ISSN~0015-5632.
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