In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances

BITTNER, Michal, Klára HILSCHEROVÁ and Giesy JOHN. In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances. Chemosphere. Oxford, UK: Elsevier Science Ltd., 2009, vol. 76, No 11, p. 1505-1508. ISSN 0045-6535.

Basic information

• Original name: In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances
• Authors: BITTNER, Michal (203 Czech Republic, guarantor), Klára HILSCHEROVÁ (203 Czech Republic) and Giesy JOHN (826 United Kingdom of Great Britain and Northern Ireland).
• Edition: Chemosphere, Oxford, UK, Elsevier Science Ltd. 2009, 0045-6535.

Other information

• Original language: English
• Type of outcome: Article in a journal
• Field of Study: 10511 Environmental sciences
• Country of publisher: United Kingdom of Great Britain and Northern Ireland
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• Impact factor: Impact factor: 3.253
• RIV identification code: RIV/00216224:14310/09:00029898
• Organization unit: Faculty of Science
• UT WoS: 000270124800009
• Keywords (in Czech): Přírodní organická hmota; Ah-recetpor; směsi; interakce
• Keywords in English: Natural organic matter; AhR; Mixture; Interaction

Abstract

Humic substances (HS) are ubiquitous natural products of decomposition of dead organic matter. HS is present in most freshwaters at concentrations ranging from 0.5 to 50 mg L-1. Organic carbon can represent 20% dry weight of sediments. Recently, the interaction of dissolved HS with the aryl hydrocarbon receptor (AhR) has been demonstrated. The AhR is a cytosolic receptor to which persistent organic pollutants (POPs) can bind and many of their toxic effects are mediated through interactions with this receptor. We describe in vitro effects (using H4IIE-luc cells) of binary mixtures of various HS with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), since in contaminated environments these compounds occur simultaneously. Six out of 12 HS samples activated AhR even at environmentally relevant concentrations (17 mg L-1), but did not reach the full AhR-activation even at excessive concentration. In simultaneous exposure of H4IIE-luc to HS (17 mg L-1) and TCDD (1.2 pM) without any preincubation prior to exposure, either significant additive or facilitative effects were observed. No negative interactions, due to possible sorption of TCDD to HS was observed. Nevertheless, if the HS-TCDD binary mixture was preincubated for 6 days prior to the exposure on H4IIE-luc cells, the additive and facilitative effects were less due to possible sorption of TCDD onto HS. Similar results were obtained from analogous experiments with greater concentrations of both TCDD and HS.

Abstract (in Czech)

Huminové látky jsou důležitou složkou v prostředí. Byly u nich prokázány i některé biologické účinky. Tato studie demonstruje intrakci huminových látek s aryl hydrocarbonovým receptorem (AhR) a jejich spolupůsobení s významným toxickým silným ligandem tohoto receptoru 2,3,7,8-tetrachlorodibenzo-p-dioxinem (TCDD).

Links

• GP525/08/P464, research and development project: Name: Interakce huminových látek a UV-VIS záření s dioxinově aktivními environmentálními polutanty

Investor: Czech Science Foundation, Interaction of humic substances and UV-VIS irradiation with dioxin-like environmental pollutants

• MSM0021622412, plan (intention): Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)

Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)