J 2009

In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances

BITTNER, Michal, Klára HILSCHEROVÁ and Giesy JOHN

Basic information

Original name

In vitro assessment of AhR-mediated activities of TCDD in mixture with humic substances

Authors

BITTNER, Michal (203 Czech Republic, guarantor), Klára HILSCHEROVÁ (203 Czech Republic) and Giesy JOHN (826 United Kingdom of Great Britain and Northern Ireland)

Edition

Chemosphere, Oxford, UK, Elsevier Science Ltd. 2009, 0045-6535

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10511 Environmental sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

Impact factor

Impact factor: 3.253

RIV identification code

RIV/00216224:14310/09:00029898

Organization unit

Faculty of Science

UT WoS

000270124800009

Keywords (in Czech)

Přírodní organická hmota; Ah-recetpor; směsi; interakce

Keywords in English

Natural organic matter; AhR; Mixture; Interaction
Změněno: 24/3/2010 11:34, RNDr. Mgr. Michal Bittner, Ph.D.

Abstract

ORIG CZ

V originále

Humic substances (HS) are ubiquitous natural products of decomposition of dead organic matter. HS is present in most freshwaters at concentrations ranging from 0.5 to 50 mg L-1. Organic carbon can represent 20% dry weight of sediments. Recently, the interaction of dissolved HS with the aryl hydrocarbon receptor (AhR) has been demonstrated. The AhR is a cytosolic receptor to which persistent organic pollutants (POPs) can bind and many of their toxic effects are mediated through interactions with this receptor. We describe in vitro effects (using H4IIE-luc cells) of binary mixtures of various HS with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), since in contaminated environments these compounds occur simultaneously. Six out of 12 HS samples activated AhR even at environmentally relevant concentrations (17 mg L-1), but did not reach the full AhR-activation even at excessive concentration. In simultaneous exposure of H4IIE-luc to HS (17 mg L-1) and TCDD (1.2 pM) without any preincubation prior to exposure, either significant additive or facilitative effects were observed. No negative interactions, due to possible sorption of TCDD to HS was observed. Nevertheless, if the HS-TCDD binary mixture was preincubated for 6 days prior to the exposure on H4IIE-luc cells, the additive and facilitative effects were less due to possible sorption of TCDD onto HS. Similar results were obtained from analogous experiments with greater concentrations of both TCDD and HS.

In Czech

Huminové látky jsou důležitou složkou v prostředí. Byly u nich prokázány i některé biologické účinky. Tato studie demonstruje intrakci huminových látek s aryl hydrocarbonovým receptorem (AhR) a jejich spolupůsobení s významným toxickým silným ligandem tohoto receptoru 2,3,7,8-tetrachlorodibenzo-p-dioxinem (TCDD).

Links

GP525/08/P464, research and development project
Name: Interakce huminových látek a UV-VIS záření s dioxinově aktivními environmentálními polutanty
Investor: Czech Science Foundation, Interaction of humic substances and UV-VIS irradiation with dioxin-like environmental pollutants
MSM0021622412, plan (intention)
Name: Interakce mezi chemickými látkami, prostředím a biologickými systémy a jejich důsledky na globální, regionální a lokální úrovni (INCHEMBIOL) (Acronym: INCHEMBIOL)
Investor: Ministry of Education, Youth and Sports of the CR, Interactions among the chemicals, environment and biological systems and their consequences on the global, regional and local scales (INCHEMBIOL)