COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE

MAŠOVÁ, Šárka, Petr WANDROL and Vlastimil BARUŠ. COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE. In Mikroskopie 2010. 2010.

Basic information

Original name

COMPARISON OF ESEM AND SEM IN MORPHOLOGY STUDIES OF PARASITIC VERTEBRATE NEMATODE

Name in Czech

Srovnání ESEM a SEM v morfologické studii paraztické hlístice

Authors

MAŠOVÁ, Šárka (203 Czech Republic, guarantor, belonging to the institution), Petr WANDROL (203 Czech Republic) and Vlastimil BARUŠ (203 Czech Republic, belonging to the institution)

Edition

Mikroskopie 2010, 2010

---

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

10600 1.6 Biological sciences

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/10:00043372

Organization unit

Faculty of Science

Keywords (in Czech)

Nematoda; ESEM; SEM; hlístice; Heterotis niloticus; Brevimulticaecum heterotis

Keywords in English

Nematoda; ESEM; SEM; nematode; Heterotis niloticus; Brevimulticaecum heterotis

Tags

Africa, Brevimulticaecum, morphology, Nematoda, nematode, SEM study, West Africa

---

Abstract

V originále

Ascaridoid nematode species Brevimulticaecum heterotis obtained by parasitological dissection from the African bonytongue, Heterotis niloticus (Osteoglossidae) collected in Senegal has been chosen for studies by scanning electron microscope to redescribe its external morphology. Scanning electron microscopy (SEM) and environmental scanning electron microscopy (ESEM) were used for the study. For SEM, specimens were fixed with hot formalin. They were dehydrated, critical-point dried, mounted on stubs and coated with gold. For ESEM, fixed specimens were hydrated with water; desiccation was not applied. Quanta 3D FEG and Quanta 250 FEG in SEM and ESEM mode were used. Combination of both techniques brings new level to nematode investigation. While SEM remains unsubstitutable method for study of nematode morphology due to its ease of use, ESEM is an unexceptionable instrument when there is only one unique specimen that cannot be destroyed or must remain available for molecular studies.

---

Links

GD526/09/H025, research and development project	Name: Evolučně-ekologická analýza společenstev a populací Investor: Czech Science Foundation, Evolutionary ecological analysis of communities and populations
LC522, research and development project	Name: ICHTYOPARAZITOLOGIE - centrum základního výzkumu Investor: Ministry of Education, Youth and Sports of the CR, Ichtyoparazitology - the center of basic research
MSM0021622416, plan (intention)	Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time