| HOLOCHOVÁ, Pavla, Vladislava RŮŽIČKOVÁ, Lucie DOSTÁLOVÁ, Roman PANTŮČEK, Petr PETRÁŠ and Jiří DOŠKAŘ. Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms. Diagnostic Microbiology and Infectious Disease. USA: Elsevier Science Inc., 2010, vol. 66, No 3, p. 248-252. ISSN 0732-8893. |
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@article{872529,
author = {Holochová, Pavla and Růžičková, Vladislava and Dostálová, Lucie and Pantůček, Roman and Petráš, Petr and Doškař, Jiří},
article_location = {USA},
article_number = {3},
keywords = {Staphylococcus aureus; Pemphigus neonatorum; Exfoliative toxin A; Prophages; Multiplex PCR},
language = {eng},
issn = {0732-8893},
journal = {Diagnostic Microbiology and Infectious Disease},
title = {Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms},
url = {http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6T60-4XNDB8V-6&_origin=SDEMFRHTML&_version=1&md5=01279d3a76e20c072c2a3c4a3d798f31},
volume = {66},
year = {2010}
}
TY - JOUR
ID - 872529
AU - Holochová, Pavla - Růžičková, Vladislava - Dostálová, Lucie - Pantůček, Roman - Petráš, Petr - Doškař, Jiří
PY - 2010
TI - Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms
JF - Diagnostic Microbiology and Infectious Disease
VL - 66
IS - 3
SP - 248-252
EP - 248-252
PB - Elsevier Science Inc.
SN - 07328893
KW - Staphylococcus aureus
KW - Pemphigus neonatorum
KW - Exfoliative toxin A
KW - Prophages
KW - Multiplex PCR
UR - http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6T60-4XNDB8V-6&_origin=SDEMFRHTML&_version=1&md5=01279d3a76e20c072c2a3c4a3d798f31
N2 - The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control. 2010 Elsevier Inc. All rights reserved.
ER -
HOLOCHOVÁ, Pavla, Vladislava RŮŽIČKOVÁ, Lucie DOSTÁLOVÁ, Roman PANTŮČEK, Petr PETRÁŠ and Jiří DOŠKAŘ. Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms. \textit{Diagnostic Microbiology and Infectious Disease}. USA: Elsevier Science Inc., 2010, vol.~66, No~3, p.~248-252. ISSN~0732-8893.
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