Rapid detection and differentiation of the exfoliative toxin
A-producing Staphylococcus aureus strains based on phiETA
prophage polymorphisms

J 2010

Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms

HOLOCHOVÁ, Pavla, Vladislava RŮŽIČKOVÁ, Lucie DOSTÁLOVÁ, Roman PANTŮČEK, Petr PETRÁŠ et. al.

Basic information

Original name

Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms

Authors

HOLOCHOVÁ, Pavla (203 Czech Republic), Vladislava RŮŽIČKOVÁ (203 Czech Republic, guarantor), Lucie DOSTÁLOVÁ (203 Czech Republic), Roman PANTŮČEK (203 Czech Republic), Petr PETRÁŠ (203 Czech Republic) and Jiří DOŠKAŘ (203 Czech Republic)

Edition

Diagnostic Microbiology and Infectious Disease, USA, Elsevier Science Inc. 2010, 0732-8893

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.426

RIV identification code

RIV/00216224:14310/10:00043374

Organization unit

Faculty of Science

UT WoS

000277577000003

Keywords in English

Staphylococcus aureus; Pemphigus neonatorum; Exfoliative toxin A; Prophages; Multiplex PCR

Abstract

ORIG CZ

V originále

The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control. 2010 Elsevier Inc. All rights reserved.

In Czech

Exfoliativní toxin A (ETA) je kódovaný genem lokalizovaným na profágu Staphylococcus aureus. V této práci jsme vyvinuli multiplex PCR reakci pro rychlou a přesnou detekci různých typů ETA-profágů serologické skupiny B odpovědných za horizontální přenos genu ETA a jeho produkci u klinických kmenů.

Links

GA310/09/0459, research and development project

Name: Úloha bakteriofágů v horizontálním přenosu genů virulence a rezistence u Staphylococcus aureus

Investor: Czech Science Foundation, Role of bacteriophages in horizontal transfer of virulence and drug resistance genes in Staphylococcus aureus

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

HOLOCHOVÁ, Pavla, Vladislava RŮŽIČKOVÁ, Lucie DOSTÁLOVÁ, Roman PANTŮČEK, Petr PETRÁŠ and Jiří DOŠKAŘ. Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms. Diagnostic Microbiology and Infectious Disease. USA: Elsevier Science Inc., 2010, vol. 66, No 3, p. 248-252. ISSN 0732-8893.

@article{872529,
   author = {Holochová, Pavla and Růžičková, Vladislava and Dostálová, Lucie and Pantůček, Roman and Petráš, Petr and Doškař, Jiří},
   article_location = {USA},
   article_number = {3},
   keywords = {Staphylococcus aureus; Pemphigus neonatorum; Exfoliative toxin A; Prophages; Multiplex PCR},
   language = {eng},
   issn = {0732-8893},
   journal = {Diagnostic Microbiology and Infectious Disease},
   title = {Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms},
   url = {http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6T60-4XNDB8V-6&_origin=SDEMFRHTML&_version=1&md5=01279d3a76e20c072c2a3c4a3d798f31},
   volume = {66},
   year = {2010}
}

TY  - JOUR
ID  - 872529
AU  - Holochová, Pavla - Růžičková, Vladislava - Dostálová, Lucie - Pantůček, Roman - Petráš, Petr - Doškař, Jiří
PY  - 2010
TI  - Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms
JF  - Diagnostic Microbiology and Infectious Disease
VL  - 66
IS  - 3
SP  - 248-252
EP  - 248-252
PB  - Elsevier Science Inc.
SN  - 07328893
KW  - Staphylococcus aureus
KW  - Pemphigus neonatorum
KW  - Exfoliative toxin A
KW  - Prophages
KW  - Multiplex PCR
UR  - http://www.sciencedirect.com/science?_ob=GatewayURL&_method=citationSearch&_uoikey=B6T60-4XNDB8V-6&_origin=SDEMFRHTML&_version=1&md5=01279d3a76e20c072c2a3c4a3d798f31
N2  - The exfoliative toxin A (ETA) is encoded by the gene located on Staphylococcus aureus prophages. We have developed a single-reaction multiplex polymerase chain reaction (PCR) assay for rapid and specific detection of various phiETA prophages of serogroup B responsible for dissemination of eta gene and ETA production in clinical strains. This PCR strategy enabled to classify the ETA-positive strains into 6 groups designated ETA-B1, ETA-B2, ETA-B3, ETA-B4, ETA-B5, and ETA-B6. The method was tested on a diverse set of 101 ETA and/or ETB-positive S. aureus strains isolated in 22 Czech maternity hospitals and 1 Slovak maternity hospital between 1998 and 2009. This novel PCR strategy is reliable in the rapid identification of yet undescribed ETA-converting B prophages and differentiation of the closely related ETA-positive strains, and it is a convenient tool for hospital epidermolytic infection control. 2010 Elsevier Inc. All rights reserved.
ER  -

HOLOCHOVÁ, Pavla, Vladislava RŮŽIČKOVÁ, Lucie DOSTÁLOVÁ, Roman PANTŮČEK, Petr PETRÁŠ and Jiří DOŠKAŘ. Rapid detection and differentiation of the exfoliative toxin A-producing Staphylococcus aureus strains based on phiETA prophage polymorphisms. \textit{Diagnostic Microbiology and Infectious Disease}. USA: Elsevier Science Inc., 2010, vol.~66, No~3, p.~248-252. ISSN~0732-8893.

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