Whole genome amplification and analysis: genome differences in the genus Treponema.

MIKALOVÁ, Lenka, Michal STROUHAL, Petra MATĚJKOVÁ POSPÍŠILOVÁ, David ŠMAJS and George M. WEINSTOCK. Whole genome amplification and analysis: genome differences in the genus Treponema. In 3rd FEMS Congress of European Microbiologists. Abstract CD, 1749. 2009.

Basic information

• Original name: Whole genome amplification and analysis: genome differences in the genus Treponema.
• Authors: MIKALOVÁ, Lenka, Michal STROUHAL, Petra MATĚJKOVÁ POSPÍŠILOVÁ, David ŠMAJS and George M. WEINSTOCK.
• Edition: 3rd FEMS Congress of European Microbiologists. Abstract CD, 1749. 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Sweden
• Confidentiality degree: is not subject to a state or trade secret
• Organization unit: Faculty of Medicine
• Keywords (in Czech): Trepomema pallidum; WGF; tpr geny
• Keywords in English: Trepomema pallidum; whole genome fingerprinting; tpr genes

Abstract

Genome comparison of 6 treponemal strains including Mexico A, DAL-1 (T. pallidum subsp. pallidum), Gauthier, CDC-2 (T. pallidum subsp. pertenue), Bosnia A (T. pallidum subsp. endemicum) and Fribourg-Blanc (unclassified simian isolate) with the sequenced genome of the syphilis spirochete TPA strain Nichols. The whole genome fingerprinting technique was used for identification of heterologous regions in the chromosomal DNA of analyzed strains. All analyzed strains contained two regions of repetitive sequences (TP0433-434, TP0470) with strain specific repeat numbers. In the yaws and simian strain genomes, three specific indels were observed. Strain specific changes comprised e. g. insertion of 58 bp (TP0136) in DAL-1, deletion of 48 bp (TP0548) and insertion of 0.5 kb (TP0695-697) in Fribourg-Blanc. Genomes of T. p. pallidum, T. p. pertenue, T. p. endemicum and unclassified simian isolate are closely related and most of the observed sequence differences are localized in tpr genes and in their vinicity.

Links

• GA310/07/0321, research and development project: Name: Komparativní genomové sekvencování patogenních treponem a transkriptomová analýza T. pallidum ssp. pallidum

Investor: Czech Science Foundation

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• NR8967, research and development project: Name: Molekulární typizace kmenů a izolátů Treponema pallidum subsp. pallidum: základ pro epidemiologickou a klinickou identifikaci jednotlivých kmenů

Investor: Ministry of Health of the CR