Detailed Information on Publication Record
2010
Rad52 SUMOylation affects the efficiency of the DNA repair
ALTMANNOVÁ, Veronika, Nadine ECKERT-BOULET, Milica ARNERIC, Peter KOLESÁR, Radka CHALOUPKOVÁ et. al.Basic information
Original name
Rad52 SUMOylation affects the efficiency of the DNA repair
Name in Czech
SUMOylace Rad52 ovlivňuje opravu DNA
Authors
ALTMANNOVÁ, Veronika (203 Czech Republic, belonging to the institution), Nadine ECKERT-BOULET (208 Denmark), Milica ARNERIC (840 United States of America), Peter KOLESÁR (203 Czech Republic, belonging to the institution), Radka CHALOUPKOVÁ (203 Czech Republic, belonging to the institution), Jiří DAMBORSKÝ (203 Czech Republic, belonging to the institution), Patrick SUNG (840 United States of America), Xiaolan ZHAO (840 United States of America), Michael LISBY (208 Denmark) and Lumír KREJČÍ (203 Czech Republic, guarantor, belonging to the institution)
Edition
Nucleic Acids Research, Oxford University Press, 2010, 0305-1048
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
10600 1.6 Biological sciences
Country of publisher
United Kingdom of Great Britain and Northern Ireland
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 7.836
RIV identification code
RIV/00216224:14310/10:00043447
Organization unit
Faculty of Science
UT WoS
000280922400022
Keywords in English
DNA repair; DNA damage; replication; genomic instability
Tags
International impact, Reviewed
Změněno: 6/1/2011 09:15, doc. Mgr. Lumír Krejčí, Ph.D.
V originále
Homologous recombination (HR) plays a vital role in DNA metabolic processes including meiosis, DNA repair, DNA replication, and rDNA homeostasis. HR defects can lead to pathological outcomes, including genetic diseases and cancer. Recent studies suggest that the post-translational modification by the small ubiquitin-like modifier (SUMO) protein plays an important role in mitotic and meiotic recombination. However, the precise role of SUMOylation during recombination is still unclear. Here, we characterize the effect of SUMOylation on the biochemical properties of the Saccharomyces cerevisiae recombination mediator protein Rad52. Interestingly, Rad52 SUMOylation is enhanced by ssDNA, and we show that SUMOylation of Rad52 also inhibits its DNA binding and annealing activities. The biochemical effects of SUMO modification in vitro are accompanied by a shorter duration of spontaneous Rad52 foci in vivo and a shift in spontaneous mitotic recombination from single-strand annealing to gene conversion events in the SUMO-deficient Rad52 mutants. Taken together, our results highlight the importance of Rad52 SUMOylation as part of a quality control mechanism regulating the efficiency of recombination and DNA repair.
In Czech
Homologní rekombinace (HR) hraje klíčovou úlohu v metabolsmu DNA, zahrnující meiósu, DNA opravu, DNA replikaci a rovnováhu rDNA. Defekty v HR vedou k patologickým výstupúm jako jsou geneticky podmíněná onemocnění a rakovina. Nedavné výsledky naznačují, že post-translační modifikace SUMOproteinem hraje důležitou roli během mitotické a meiotické rekombinaci. Nicméně přesná úloha SUMOylace během rekombinace neni doposud objasněna. Charakterizovali jsme efekt SUMOylaca na biochemické vlastnosti proteinu Rad52.
Links
| GA301/09/1917, research and development project |
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| GD203/09/H046, research and development project |
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| LC06030, research and development project |
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| ME10048, research and development project |
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| MSM0021622413, plan (intention) |
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