KAMAL, Sharaf, Lucie HOROVÁ, Tomáš PAVLÍČEK, Nevo EVIATAR and Petr BUREŠ. Genome size and base composition in Oryzaephilus surinamensis (Coleoptera: Sylvanidae) and differences between native (feral) and silo pest populations in Israel. Journal of Stored Products Research. Amsterdam, New York: Elsevier, 2010, vol. 46, No 1, p. 34–37. ISSN 0022-474X.
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Basic information
Original name Genome size and base composition in Oryzaephilus surinamensis (Coleoptera: Sylvanidae) and differences between native (feral) and silo pest populations in Israel
Name in Czech Velikost genomu a genomický poměr bází u Oryzaephilus surinamensis (Coleoptera: Sylvanidae): rozdíly mezi přírodními a skladištními populacemi v Izraeli
Authors KAMAL, Sharaf (376 Israel), Lucie HOROVÁ (203 Czech Republic, belonging to the institution), Tomáš PAVLÍČEK (376 Israel), Nevo EVIATAR (376 Israel) and Petr BUREŠ (203 Czech Republic, guarantor, belonging to the institution).
Edition Journal of Stored Products Research, Amsterdam, New York, Elsevier, 2010, 0022-474X.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.438
RIV identification code RIV/00216224:14310/10:00043493
Organization unit Faculty of Science
UT WoS 000276115400006
Keywords (in Czech) velikost genomu; AT/CG; Oryzaephilus surinamensis; Wolbachia
Keywords in English Genome size; AT/CG; Oryzaephilus surinamensis; Wolbachia
Tags International impact, Reviewed
Changed by Changed by: prof. RNDr. Petr Bureš, Ph.D., učo 2635. Changed: 29/4/2013 12:08.
Abstract
In this study, flow cytometry was used for assessing and comparing the genome size (GS) and the whole genome base composition (AT/GC ratio) of the saw toothed grain beetle Oryzaephilus surinamensis (L.). In addition, the presence and frequency of endosymbiotic Wolbachia bacteria was studied. The haploid GS was estimated to lie within the range from 151.5 to 154 Mbp in O. surinamensis, making it the smallest value of haploid GS known among beetles. Furthermore, it was found that in eight silo pest populations GS was significantly smaller than in eight feral (native) populations obtained from fallen oak acorns. The ability of O. surinamensis to colonize different habitats globally could be connected with an unusually ATrich (for an invertebrate) genome (ATbase content ranging from 68 to 76%). Native (feral) populations of O. surinamensis appear to have genetically diverged from the storage-pest populations tested.
Abstract (in Czech)
Průtoková cytometrie byla použita pro stanovení a srovnání velikosti genomu a genomové složení bazí (poměr AT/GC) u skladištního brouka Oryzaephilus surinamensis (L.). V trávicím traktu tohoto brouka byla dále studována přítomnost endosymbiotické bakterie Wolbachia. Srovnáním s interními standardami s odpovídající velikostí genomu stanovenou sekvenováním (Oryza sativa subsp. japonica Nipponbare, Arabidopsis thaliana Columbia, samičí linie Drosophila melanogaster) byla haploidní velikost genomu stanovena v rozmezí od 151.5 do 154 Mbp u studovaného O. surinamensis. Tato velikost je dosud nejmenší známou velikostí u brouků (Coleoptera). U osmi skladištních populací byla velikost genomu signifikantně menší než u osmi přirozených populací živících se dubovými žaludy. Schopnost O. surinamensis kolonizovat různé habitaty může být spojena s neobvykle AT bohatým (ve srovnání s jinými bezobratlými) genomem (obsah AT-bazí kolísá od 68 do 76%). Přirozané (ferální) populace O. surinamensis jsou více geneticky diverzifikované než populace skladištní.
Links
LC06073, research and development projectName: Centrum pro výzkum biodiverzity
Investor: Ministry of Education, Youth and Sports of the CR, Biodiversity Research Center
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
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