Detailed Information on Publication Record
2010
Haplotype analysis of interleukin 18 (IL-18) gene in Czech patients with allergic disorders
IZAKOVIČOVÁ HOLLÁ, Lydie, Barbara HRDLIČKOVÁ, Marcel SCHÜLLER, Dana BUČKOVÁ, Dagmar KINDLOVÁ et. al.Basic information
Original name
Haplotype analysis of interleukin 18 (IL-18) gene in Czech patients with allergic disorders
Name in Czech
Haplotypová analýza genu pro interleukin 18 (IL-18) u českých pacientů s alergickým onemocněním
Authors
IZAKOVIČOVÁ HOLLÁ, Lydie (203 Czech Republic, guarantor, belonging to the institution), Barbara HRDLIČKOVÁ (203 Czech Republic, belonging to the institution), Marcel SCHÜLLER (203 Czech Republic), Dana BUČKOVÁ (203 Czech Republic), Dagmar KINDLOVÁ (203 Czech Republic, belonging to the institution), Vincent IZAKOVIČ (203 Czech Republic) and Anna VAŠKŮ (203 Czech Republic, belonging to the institution)
Edition
Human Immunology, USA, ELSEVIER SCIENCE INC, 2010, 0198-8859
Other information
Language
English
Type of outcome
Článek v odborném periodiku
Field of Study
30102 Immunology
Country of publisher
United States of America
Confidentiality degree
není předmětem státního či obchodního tajemství
Impact factor
Impact factor: 2.872
RIV identification code
RIV/00216224:14110/10:00043500
Organization unit
Faculty of Medicine
UT WoS
000278426100009
Keywords (in Czech)
alergické nemoci; astma; IL-18; haplotyp; polymorfizmus
Keywords in English
allergic diseases; asthma; IL-18; haplotype; polymorphism
Tags
International impact
Změněno: 8/10/2012 20:18, Mgr. Michal Petr
V originále
The interleukin-18 (IL-18) gene on chromosome 11q22 has been suggested as a susceptibility factor for allergies. To test for a possible role of IL-18 polymorphisms in Czech population, case-control study including 958 subjects (633 allergic patients and 325 healthy controls) was performed. An allele-specific polymerase chain reaction was used to analyze variants at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the promoter region together with the polymerase chain reaction-restriction fragment length polymorphism method for the detection of polymorphism at position -140 C/G (previously -133 C/G, rs360721) in intron 1 of the IL-18 gene. The -1297 C/T (rs360719) polymorphism was genotyped by real-time-polymerase chain reaction, using a predevelopment TaqMan allele discrimination assay. There were no significant differences in distribution of alleles or genotypes in any of four single nucleotide polymorphisms in the IL-18 gene between controls and patients. However, subsequent analysis revealed a significant difference in haplotype frequencies between the allergic patients and healthy subjects (p < 0.01). Haplotype formed by -1297 C/-607 A/-137 C/-140 C alleles occurred significantly more frequently in patients than controls (0.0433 vs 0.0129; p < 0.0003; p(corr)< 0.01, OR = 3.37; 95% CI = 1.59-7.14). In contrast, there was no relationship among the IL-18 variants and total serum IgE level. Our results indicate that promoter polymorphisms in the IL-18 gene act in interaction and could play a role in allergic disorders.
In Czech
Gen pro interleukin-18 (IL-18)na chromozomu 11q22 je jedním z "kandidátní" genů pro alergická onemocnění. V práci jsme analyzovali polymorfizmy v genu pro IL-18 v české populaci s použitím přístupu "kontroly-případy" u 958 osob (633 alergických pacientů a 325 kontrol. Naše výsledky naznačují interakci mezi polymorfizmy v genu pro IL-18 a jejich úlohu při rozvoji alergických chorob.
Links
| GA310/06/0827, research and development project |
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| 2B06060, research and development project |
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