| IZAKOVIČOVÁ HOLLÁ, Lydie, Barbara HRDLIČKOVÁ, Marcel SCHÜLLER, Dana BUČKOVÁ, Dagmar KINDLOVÁ, Vincent IZAKOVIČ and Anna VAŠKŮ. Haplotype analysis of interleukin 18 (IL-18) gene in Czech patients with allergic disorders. Human Immunology. USA: ELSEVIER SCIENCE INC, 2010, vol. 71, No 6, p. 592-597. ISSN 0198-8859. Available from: https://dx.doi.org/10.1016/j.humimm.2010.03.004. |
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@article{875850,
author = {Izakovičová Hollá, Lydie and Hrdličková, Barbara and Schüller, Marcel and Bučková, Dana and Kindlová, Dagmar and Izakovič, Vincent and Vašků, Anna},
article_location = {USA},
article_number = {6},
doi = {http://dx.doi.org/10.1016/j.humimm.2010.03.004},
keywords = {allergic diseases; asthma; IL-18; haplotype; polymorphism},
language = {eng},
issn = {0198-8859},
journal = {Human Immunology},
title = {Haplotype analysis of interleukin 18 (IL-18) gene in Czech patients with allergic disorders},
volume = {71},
year = {2010}
}
TY - JOUR
ID - 875850
AU - Izakovičová Hollá, Lydie - Hrdličková, Barbara - Schüller, Marcel - Bučková, Dana - Kindlová, Dagmar - Izakovič, Vincent - Vašků, Anna
PY - 2010
TI - Haplotype analysis of interleukin 18 (IL-18) gene in Czech patients with allergic disorders
JF - Human Immunology
VL - 71
IS - 6
SP - 592-597
EP - 592-597
PB - ELSEVIER SCIENCE INC
SN - 01988859
KW - allergic diseases
KW - asthma
KW - IL-18
KW - haplotype
KW - polymorphism
N2 - The interleukin-18 (IL-18) gene on chromosome 11q22 has been suggested as a susceptibility factor for allergies. To test for a possible role of IL-18 polymorphisms in Czech population, case-control study including 958 subjects (633 allergic patients and 325 healthy controls) was performed. An allele-specific polymerase chain reaction was used to analyze variants at positions -607 C/A (rs1946518) and -137 G/C (rs187238) in the promoter region together with the polymerase chain reaction-restriction fragment length polymorphism method for the detection of polymorphism at position -140 C/G (previously -133 C/G, rs360721) in intron 1 of the IL-18 gene. The -1297 C/T (rs360719) polymorphism was genotyped by real-time-polymerase chain reaction, using a predevelopment TaqMan allele discrimination assay. There were no significant differences in distribution of alleles or genotypes in any of four single nucleotide polymorphisms in the IL-18 gene between controls and patients. However, subsequent analysis revealed a significant difference in haplotype frequencies between the allergic patients and healthy subjects (p < 0.01). Haplotype formed by -1297 C/-607 A/-137 C/-140 C alleles occurred significantly more frequently in patients than controls (0.0433 vs 0.0129; p < 0.0003; p(corr)< 0.01, OR = 3.37; 95% CI = 1.59-7.14). In contrast, there was no relationship among the IL-18 variants and total serum IgE level. Our results indicate that promoter polymorphisms in the IL-18 gene act in interaction and could play a role in allergic disorders.
ER -
IZAKOVIČOVÁ HOLLÁ, Lydie, Barbara HRDLIČKOVÁ, Marcel SCHÜLLER, Dana BUČKOVÁ, Dagmar KINDLOVÁ, Vincent IZAKOVIČ and Anna VAŠKŮ. Haplotype analysis of interleukin 18 (IL-18) gene in Czech patients with allergic disorders. \textit{Human Immunology}. USA: ELSEVIER SCIENCE INC, 2010, vol.~71, No~6, p.~592-597. ISSN~0198-8859. Available from: https://dx.doi.org/10.1016/j.humimm.2010.03.004.
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