J 2009

Interaction of Cytidine 5 '-Monophosphate with Au(111): An In Situ Infrared Spectroscopic Study

DONEUX, Thomas and Lukáš FOJT

Basic information

Original name

Interaction of Cytidine 5 '-Monophosphate with Au(111): An In Situ Infrared Spectroscopic Study

Authors

DONEUX, Thomas and Lukáš FOJT

Edition

ChemPhysChem, Weinheim, WILEY-VCH Verlag GmbH & Co. KGaA, 2009, 1439-4235

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10610 Biophysics

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 3.453

Organization unit

Faculty of Medicine

UT WoS

000267928100043

Keywords in English

chemisorption; electrochemistry; interfaces; IR spectroscopy; nucleobases

Tags

Tags

International impact, Reviewed
Změněno: 24/3/2010 10:41, Mgr. Lukáš Fojt, Ph.D.

Abstract

V originále

The interaction of cytidine 5-monophosphate (CMP) with gold surfaces is studied by means of in situ infrared spectroscopy and cyclic voltammetry at the Au(111)/aqueous solution interface.Similar to other nucleic acid components, cytidine 5-monophosphate is chemisorbed on the surface at positive potentials, and the amount of adsorbed CMP increases with the potential. Subtractively normalized interfacial Fourier-transform infrared spectroscopy (SNIFTIRS) is used to identify the adsorbed and desorbed species. Upon electrochemical desorption, the molecules released in solution are unprotonated on the N3 atom. Striking similarities are found between the spectrum of adsorbed CMP and the solution spectrum of protonated CMP. The origin of such similarities is discussed. The results strongly suggest that chemisorption occurs through the N3 atom of the pyrimidine ring. A comparison is drawn with cytidine, whose electrochemical and spectroscopic behaviors are also investigated.