Identification of genes participating in the regulation of CKI1 expression using forward genetic approach

DOBISOVÁ, Tereza and Jan HEJÁTKO. Identification of genes participating in the regulation of CKI1 expression using forward genetic approach. In Auxins and Cytokinins in Plant Development International symposium (ACPD 2009), July 10-14, Prague, Czech Republic. 2009.

Basic information

• Original name: Identification of genes participating in the regulation of CKI1 expression using forward genetic approach
• Name in Czech: Identifikace genů podílejících se na regulaci exprese genu CKI1 za užití přístupu přímé genetiky
• Authors: DOBISOVÁ, Tereza (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution).
• Edition: Auxins and Cytokinins in Plant Development International symposium (ACPD 2009), July 10-14, Prague, Czech Republic, 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/09:00029985
• Organization unit: Faculty of Science
• Keywords (in Czech): CKI1; přímá genetika, EMS mutageneze
• Keywords in English: CKI1; forward genetic; EMS mutagenesis

Abstract

Overexpression of a hybrid histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling pathway and cytokinin-like response in Arabidopsis hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of twocomponent signaling. We used forward genetics approach to identify the factors regulating the activity of CKI1 promoter. Stable transgenic lines carrying ProCKI1:uidA construct were mutated using EMS and M2 seedlings were screened for changes in the GUS pattern. To eliminate constraints of the rate-limiting factor that is a microscopy of the screened mutant lines, we have adopted automated microscopy using the dotSlide system. The level of CKI1 gene expression in mutant lines was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of selected mutant lines and the influence of spatiotemporal changes in CKI1 expression on the two-component signalling will be analyzed and affected genes will be identified using map-based cloning. (Supported by MSM0021622415, LC06034 and 204/08/H054)

Links

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations