IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH

DOBISOVÁ, Tereza and Jan HEJÁTKO. IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH. In 7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us". 2009. ISBN 978-80-7375-310-8.

Basic information

• Original name: IDENTIFICATION OF GENES PARTICIPATING IN THE REGULATION OF CKI1 EXPRESION USING FORWARD GENETIC APPROACH
• Authors: DOBISOVÁ, Tereza (203 Czech Republic, belonging to the institution) and Jan HEJÁTKO (203 Czech Republic, guarantor, belonging to the institution).
• Edition: 7th International Conference of Ph.D Students on Experimental Plant Biology "News - what the plants told us" 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• WWW: URL
• RIV identification code: RIV/00216224:14310/09:00043563
• Organization unit: Faculty of Science
• ISBN: 978-80-7375-310-8
• Keywords (in Czech): CKI1; EMS mutageneze; přímá genetika
• Keywords in English: CKI1; EMS mutagenesis; forward genetics

Abstract

Overexpression of the histidine kinase CKI1 results into cytokinin-independent activation of two-component signaling (TCS) pathway and cytokinin-like response in hypocotyl explants. CKI1 was found to be involved in the female gametophyte development and procambium activity during vascular tissue formation. Recent data suggest that regulation of CKI1 expression might contribute to the regulation of TCS. We used forward genetics approach to identify the factors regulating the activity of CKI1 promotor. Transgenic lines carrying ProCKI1:uidA construct were mutated by EMS and M2 seedlings were screened for changes in the GUS pattern using automated microscopy (dotSlide) system. The level of CKI1 gene expression in mutants was tested using qRT-PCR and the more detailed analysis of selected mutants is in the progress. Phenotype of these mutants and the influence of spatiotemporal changes in CKI1 expression on the TCS will be analyzed and affected genes will be identified using map-based cloning.

Links

• GD204/08/H054, research and development project: Name: Molekulární mechanismy proliferace a diferenciace buněk

Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations