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@inproceedings{877325, author = {Musilová, Jindra and Glatz, Zdeněk}, address = {Brno, Czech Republic}, booktitle = {Final Program and Book of Abstracts}, edition = {první}, keywords = {Metabolom-purine and pyrimidine nucleotides-adenine coenzymes-capillary electrophoresis}, language = {eng}, location = {Brno, Czech Republic}, isbn = {978-80-254-6631-5}, pages = {114-114}, publisher = {Institute of Analytical Chemistry AS CR, v.v.i.}, title = {Microbial targeted metabolome analysis of common nucleotides and coenzymes by capillary electrophoresis}, year = {2010} }
TY - JOUR ID - 877325 AU - Musilová, Jindra - Glatz, Zdeněk PY - 2010 TI - Microbial targeted metabolome analysis of common nucleotides and coenzymes by capillary electrophoresis PB - Institute of Analytical Chemistry AS CR, v.v.i. CY - Brno, Czech Republic SN - 9788025466315 KW - Metabolom-purine and pyrimidine nucleotides-adenine coenzymes-capillary electrophoresis N2 - The quantitative determination of nucleotides is important in many fields of biochemical research. Intracellular level of nucleotides characterizes the energetic state of the cell under a variation of physiological conditions during the cell growth. The main aim of this work was to optimize conditions for selective and rapid determination of 17 energetically important metabolites (purine and pyrimidine nucleotides, adenine coenzymes and Acetyl CoA) using capillary electrophoresis (CE). Because of low intra- and extracellular concentration of these metabolites, the capillary zone electrophoresis was combined with the on-line preconcentration technique - field enhanced sample stacking - to improve the concentration sensitivity. Optimized method was used for analyzing of cell-extract of bacterium Paracoccus denitrificans. ER -
MUSILOVÁ, Jindra a Zdeněk GLATZ. Microbial targeted metabolome analysis of common nucleotides and coenzymes by capillary electrophoresis. In \textit{Final Program and Book of Abstracts}. první. Brno, Czech Republic: Institute of Analytical Chemistry AS CR, v.v.i. s.~114. ISBN~978-80-254-6631-5. 2010.
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