a 2009

Recognition of phosphorylated CTD of RNA Pol II by Nrd1

ČERNÁ, Hana, Karel KUBÍČEK and Richard ŠTEFL

Basic information

Original name

Recognition of phosphorylated CTD of RNA Pol II by Nrd1

Name in Czech

Recognition of phosphorylated CTD of RNA Pol II by Nrd1

Authors

ČERNÁ, Hana (203 Czech Republic), Karel KUBÍČEK (203 Czech Republic) and Richard ŠTEFL (203 Czech Republic, guarantor)

Edition

Recognition of phosphorylated CTD of RNA Pol II by Nrd1, 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00028837

Organization unit

Faculty of Science

ISSN

Keywords in English

NMR; CTD; RNA; transcription termination
Změněno: 25/3/2010 18:14, prof. Mgr. Richard Štefl, Ph.D.

Abstract

V originále

The transcription by RNA polymerase II (Pol II) in Saccharomyces cerevisiae can be terminated via at least two pathways - the mRNA pathway and ncRNA pathway. Both of them require interaction of CTD domain of RNA poly II with CID domain of a specialized protein. The CTD domain of RNA polymerase II contains tandem repeats of a heptad sequence that are dynamically phosphorylated or dephosphorylated on Ser5 and Ser2 over the course of transcription. The Nrd1 protein acts in ncRNA pathway - where Nrd1 (in complex with Nab3 and Sen1) is via its CID domain bound to CTD domain of RNA poly II. Depending on the extent of exosome degradation, this termination pathway can lead to either 3' end trimming or complete degradation. Unlike other factors of transcription termination, Nrd1 binds preferentially to CTD phosphorylated at Ser5, which has the highest levels of fosforylation in early elongation. We will present our NMR study of the Nrd1 CTD domain and its interaction with the Ser5-phosphorylated CTD of RNA Pol II.

Links

GA204/08/1212, research and development project
Name: Strukturní studium interakcí mezi proteiny a RNA účastnící se v mechanismu kontroly kvality RNA
Investor: Czech Science Foundation, Structural studies of protein-RNA complexes involved in RNA quality control
IAA401630903, research and development project
Name: Strukturní podstata mechanismu ukončení transkripce nepolyadenylovaných transkriptů
Investor: Academy of Sciences of the Czech Republic, Structural basis for transcription termination of nonpolyadenylated transcripts
LA08008, research and development project
Name: Strukturní studium interakcí mezi bíkovinami a poškozenou RNA.
Investor: Ministry of Education, Youth and Sports of the CR, Structural studies of protein-RNA complexes involved in RNA quality control
MSM0021622413, plan (intention)
Name: Proteiny v metabolismu a při interakci organismů s prostředím
Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment