Structure and RNA binding of Nab3

HÓBOR, Fruzsina, Karel KUBÍČEK, Josef PASULKA and Richard ŠTEFL. Structure and RNA binding of Nab3. In Structure and RNA binding of Nab3. 2009. ISSN 1211-5894.

Basic information

• Original name: Structure and RNA binding of Nab3
• Authors: HÓBOR, Fruzsina (348 Hungary), Karel KUBÍČEK (203 Czech Republic), Josef PASULKA (203 Czech Republic) and Richard ŠTEFL (203 Czech Republic, guarantor).
• Edition: Structure and RNA binding of Nab3, 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/09:00028838
• Organization unit: Faculty of Science
• ISSN: 1211-5894
• Keywords in English: NMR; Nab3; RNA binding ; transcription termination; structure

Abstract

Besides mRNA, RNA polymerase II also transcribes a subset of small nuclear and small nucleoar RNAs, and a class of intergenic and anti-sense RNAs. Termination of these transcripts requires the nuclear pre-mRNA down- regulation (Nrd)1 and the nuclear polyadenylated RNA-binding (Nab)3 proteins, and the RNA helicase Sen1. In this so-called nonpoly(A) termination pathway, the RNA-binding proteins, Nrd1 and Nab3, recognize Nrd1- and Nab3-binding sites which is an initial step in the termination and subsequent processing or degradation of these transcripts. Recent studies identified sequence motives for Nrd1 and Nab3, as the signals that direct termination and exosome-TRAMP trimming/degradation of nonpolyadenylated transcripts. It was shown that the RNA-recognition motif (RRM) of Nrd1 and Nab3 bind to the GUA[A/G] and UCUU sequences, respectively. In addition, it was demonstrated that Nrd1 and Nab3 form a stable heterodimer and bind to snoRNA terminators that contain multiple Nrd1- and Nab3- binding sequences. To fully understand the structural basis behind the RNA recognition by the Nrd1-Nab3 complex, we use multidimensional NMR spectroscopy to determine the three- dimensional structures of the individual RRMs of Nrd1 and Nab3 and the minimal Nrd1- Nab3 heterodimer alone as well as in complex with their RNA substrates.

Links

• GA204/08/1212, research and development project: Name: Strukturní studium interakcí mezi proteiny a RNA účastnící se v mechanismu kontroly kvality RNA

Investor: Czech Science Foundation, Structural studies of protein-RNA complexes involved in RNA quality control

• IAA401630903, research and development project: Name: Strukturní podstata mechanismu ukončení transkripce nepolyadenylovaných transkriptů

Investor: Academy of Sciences of the Czech Republic, Structural basis for transcription termination of nonpolyadenylated transcripts

• LA08008, research and development project: Name: Strukturní studium interakcí mezi bíkovinami a poškozenou RNA.

Investor: Ministry of Education, Youth and Sports of the CR, Structural studies of protein-RNA complexes involved in RNA quality control

• MSM0021622413, plan (intention): Name: Proteiny v metabolismu a při interakci organismů s prostředím

Investor: Ministry of Education, Youth and Sports of the CR, Proteins in metabolism and interaction of organisms with the environment