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@proceedings{877489,
author = {Preisler, Jan and Jungová, Pavla and Peš, Ondřej and Kanický, Viktor and Navrátilová, Jarmila and Šmarda, Jan and Krásenský, Pavel and Vaculovič, Tomáš},
booktitle = {25th International Symposium on MicroScale Bioseparations MSB 2010},
keywords = {off-line coupling; microcolumn separation; capillary electrophoresis; mass spectrometry; MALDI; ICP; LIF; proteins},
language = {eng},
isbn = {978-80-254-6631-5},
title = {A Multidetection Platform for Microcolumn Separations},
year = {2010}
}
TY - CONF
ID - 877489
AU - Preisler, Jan - Jungová, Pavla - Peš, Ondřej - Kanický, Viktor - Navrátilová, Jarmila - Šmarda, Jan - Krásenský, Pavel - Vaculovič, Tomáš
PY - 2010
TI - A Multidetection Platform for Microcolumn Separations
SN - 9788025466315
KW - off-line coupling
KW - microcolumn separation
KW - capillary electrophoresis
KW - mass spectrometry
KW - MALDI
KW - ICP
KW - LIF
KW - proteins
N2 - An instrumental platform based on a universal off-line interface for deposition of capillary electrophoresis (CE) or liquid chromatography (LC) effluent on a suitable target will be described. The deposited fractions of analytes, such as peptides, proteins, metalloproteins or inorganic compounds may be analyzed in off-line mode using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Laser-induced fluorescence (LIF) detection may be used for visualization of analytes on target. Analytes may be subjected to reactions on target, such as digestion for protein identification. Additionally, inductively-coupled plasma mass spectrometry (ICP MS) detection mode, nature of which is complementary to soft MALDI MS, may be exploited.
ER -
PREISLER, Jan, Pavla JUNGOVÁ, Ondřej PEŠ, Viktor KANICKÝ, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA, Pavel KRÁSENSKÝ a Tomáš VACULOVIČ. A Multidetection Platform for Microcolumn Separations. In \textit{25th International Symposium on MicroScale Bioseparations MSB 2010}. ISBN~978-80-254-6631-5. 2010.
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