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@proceedings{877489, author = {Preisler, Jan and Jungová, Pavla and Peš, Ondřej and Kanický, Viktor and Navrátilová, Jarmila and Šmarda, Jan and Krásenský, Pavel and Vaculovič, Tomáš}, booktitle = {25th International Symposium on MicroScale Bioseparations MSB 2010}, keywords = {off-line coupling; microcolumn separation; capillary electrophoresis; mass spectrometry; MALDI; ICP; LIF; proteins}, language = {eng}, isbn = {978-80-254-6631-5}, title = {A Multidetection Platform for Microcolumn Separations}, year = {2010} }
TY - CONF ID - 877489 AU - Preisler, Jan - Jungová, Pavla - Peš, Ondřej - Kanický, Viktor - Navrátilová, Jarmila - Šmarda, Jan - Krásenský, Pavel - Vaculovič, Tomáš PY - 2010 TI - A Multidetection Platform for Microcolumn Separations SN - 9788025466315 KW - off-line coupling KW - microcolumn separation KW - capillary electrophoresis KW - mass spectrometry KW - MALDI KW - ICP KW - LIF KW - proteins N2 - An instrumental platform based on a universal off-line interface for deposition of capillary electrophoresis (CE) or liquid chromatography (LC) effluent on a suitable target will be described. The deposited fractions of analytes, such as peptides, proteins, metalloproteins or inorganic compounds may be analyzed in off-line mode using matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). Laser-induced fluorescence (LIF) detection may be used for visualization of analytes on target. Analytes may be subjected to reactions on target, such as digestion for protein identification. Additionally, inductively-coupled plasma mass spectrometry (ICP MS) detection mode, nature of which is complementary to soft MALDI MS, may be exploited. ER -
PREISLER, Jan, Pavla JUNGOVÁ, Ondřej PEŠ, Viktor KANICKÝ, Jarmila NAVRÁTILOVÁ, Jan ŠMARDA, Pavel KRÁSENSKÝ and Tomáš VACULOVIČ. A Multidetection Platform for Microcolumn Separations. In \textit{25th International Symposium on MicroScale Bioseparations MSB 2010}. 2010. ISBN~978-80-254-6631-5.
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