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@proceedings{877642,
author = {Hrdinová, Vendula and Hejátko, Jan and Horák, Jakub},
booktitle = {5. Metodické dny},
keywords = {two-component system; signal transduction; protein-protein interaction; BiFC; yeast two-hybrid; histidine kinase; AHP; Arabidopsis},
language = {eng},
title = {Interactions of the receiver domain in histidine kinase receptors with the AHP proteins},
year = {2009}
}
TY - CONF
ID - 877642
AU - Hrdinová, Vendula - Hejátko, Jan - Horák, Jakub
PY - 2009
TI - Interactions of the receiver domain in histidine kinase receptors with the AHP proteins
KW - two-component system
KW - signal transduction
KW - protein-protein interaction
KW - BiFC
KW - yeast two-hybrid
KW - histidine kinase
KW - AHP
KW - Arabidopsis
N2 - Two-component systems belong to important sensing / response mechanisms in higher plants. AHP proteins interact with histidine kinase receptors as well as with response regulators, and therefore link the signal recognition by membrane sensors with regulation of transcription via nuclear-located transcription factors that mediate plant responses to the environmental stimuli. In our study the interactions of CKI1 receiver domain with all six AHP proteins were analyzed in vivo. Using the yeast two-hybrid system we revealed that CKI1 receiver domain interacts with AHP2, AHP3 and AHP5, but not with AHP1, AHP4 and AHP6. In addition, bimolecular fluorescence complementation analysis (BiFC) was carried out in transiently transformed tobacco leaves and the interaction pattern was confirmed. Combining these two methods for analysis of protein-protein interactions in vivo we gained a rapid insight into the specificity of elements involved in a signalling cascade downstream of CKI1
ER -
HRDINOVÁ, Vendula, Jan HEJÁTKO a Jakub HORÁK. Interactions of the receiver domain in histidine kinase receptors with the AHP proteins. In \textit{5. Metodické dny}. 2009. ISSN~1213-6670.
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