a 2009

Interactions of the receiver domain in histidine kinase receptors with the AHP proteins

HRDINOVÁ, Vendula, Jan HEJÁTKO and Jakub HORÁK

Basic information

Original name

Interactions of the receiver domain in histidine kinase receptors with the AHP proteins

Authors

HRDINOVÁ, Vendula (203 Czech Republic), Jan HEJÁTKO (203 Czech Republic) and Jakub HORÁK (203 Czech Republic, guarantor)

Edition

5. Metodické dny, 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00029998

Organization unit

Faculty of Science

ISSN

Keywords (in Czech)

dvoukomponentní systém; signální dráhy; protei-proteinové interakce; histidin kinázy; AHP; Arabidopsis

Keywords in English

two-component system; signal transduction; protein-protein interaction; BiFC; yeast two-hybrid; histidine kinase; AHP; Arabidopsis
Změněno: 26/3/2010 16:28, Mgr. Jakub Horák, Dr. rer. nat.

Abstract

V originále

Two-component systems belong to important sensing / response mechanisms in higher plants. AHP proteins interact with histidine kinase receptors as well as with response regulators, and therefore link the signal recognition by membrane sensors with regulation of transcription via nuclear-located transcription factors that mediate plant responses to the environmental stimuli. In our study the interactions of CKI1 receiver domain with all six AHP proteins were analyzed in vivo. Using the yeast two-hybrid system we revealed that CKI1 receiver domain interacts with AHP2, AHP3 and AHP5, but not with AHP1, AHP4 and AHP6. In addition, bimolecular fluorescence complementation analysis (BiFC) was carried out in transiently transformed tobacco leaves and the interaction pattern was confirmed. Combining these two methods for analysis of protein-protein interactions in vivo we gained a rapid insight into the specificity of elements involved in a signalling cascade downstream of CKI1

Links

GD204/08/H054, research and development project
Name: Molekulární mechanismy proliferace a diferenciace buněk
Investor: Czech Science Foundation, Molecular mechanisms of the cell proliferation and differentiation
LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations