Detailed Information on Publication Record
2009
Interactions of the receiver domain in histidine kinase receptors with the AHP proteins
HRDINOVÁ, Vendula, Jan HEJÁTKO and Jakub HORÁKBasic information
Original name
Interactions of the receiver domain in histidine kinase receptors with the AHP proteins
Authors
HRDINOVÁ, Vendula (203 Czech Republic), Jan HEJÁTKO (203 Czech Republic) and Jakub HORÁK (203 Czech Republic, guarantor)
Edition
5. Metodické dny, 2009
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/09:00029998
Organization unit
Faculty of Science
ISSN
Keywords (in Czech)
dvoukomponentní systém; signální dráhy; protei-proteinové interakce; histidin kinázy; AHP; Arabidopsis
Keywords in English
two-component system; signal transduction; protein-protein interaction; BiFC; yeast two-hybrid; histidine kinase; AHP; Arabidopsis
Změněno: 26/3/2010 16:28, Mgr. Jakub Horák, Dr. rer. nat.
Abstract
V originále
Two-component systems belong to important sensing / response mechanisms in higher plants. AHP proteins interact with histidine kinase receptors as well as with response regulators, and therefore link the signal recognition by membrane sensors with regulation of transcription via nuclear-located transcription factors that mediate plant responses to the environmental stimuli. In our study the interactions of CKI1 receiver domain with all six AHP proteins were analyzed in vivo. Using the yeast two-hybrid system we revealed that CKI1 receiver domain interacts with AHP2, AHP3 and AHP5, but not with AHP1, AHP4 and AHP6. In addition, bimolecular fluorescence complementation analysis (BiFC) was carried out in transiently transformed tobacco leaves and the interaction pattern was confirmed. Combining these two methods for analysis of protein-protein interactions in vivo we gained a rapid insight into the specificity of elements involved in a signalling cascade downstream of CKI1
Links
GD204/08/H054, research and development project |
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LC06034, research and development project |
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MSM0021622415, plan (intention) |
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