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@article{878164,
author = {Bouchal, Pavel and Struhárová, Iva and Budinská, Eva and Šedo, Ondrej and Vyhlídalová, Tereza and Zdráhal, Zbyněk and van Spanning, Rob and Kučera, Igor},
article_location = {Amsterdam, The Netherlands},
article_number = {6},
doi = {http://dx.doi.org/10.1016/j.bbapap.2010.01.016},
keywords = {Paracoccus denitrificans; FNR transcription regulator; Respiratory switch; Two-dimensional gel electrophoresis; Proteome},
language = {eng},
issn = {1570-9639},
journal = {Biochimica et Biophysica Acta - Proteins and Proteomics},
title = {Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach},
url = {http://dx.doi.org/10.1016/j.bbapap.2010.01.016},
volume = {1804},
year = {2010}
}
TY - JOUR
ID - 878164
AU - Bouchal, Pavel - Struhárová, Iva - Budinská, Eva - Šedo, Ondrej - Vyhlídalová, Tereza - Zdráhal, Zbyněk - van Spanning, Rob - Kučera, Igor
PY - 2010
TI - Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
VL - 1804
IS - 6
SP - 1309-1316
EP - 1309-1316
PB - Elsevier
SN - 15709639
KW - Paracoccus denitrificans
KW - FNR transcription regulator
KW - Respiratory switch
KW - Two-dimensional gel electrophoresis
KW - Proteome
UR - http://dx.doi.org/10.1016/j.bbapap.2010.01.016
N2 - The switch from aerobic to anaerobic respiration in the bacterium Paracoccus denitrificans is orchestrated by the action of three FNR-type transcription regulators FnrP, NNR and NarR, which are sensors for oxygen, nitric oxide and nitrite, respectively. In this work, we analyzed the protein composition of four strains (wild type, FnrP-, NNR- and NarR-mutant strains) grown aerobically, semiaerobically and semiaerobically in the presence of nitrate to discover the global role of FNR-family transcription regulators using proteomics, with data validation at the transcript and genome levels. Expression profiles were acquired using two-dimensional gel electrophoresis for 737 protein spots, in which 640 proteins were identified using mass spectrometry. The annotated 2-D proteome map provided the most comprehensive coverage of P. denitrificans proteome available to-date and can be accessed on-line at http://www.mpiib-berlin.mpg.de/2D-PAGE/. Our results revealed several new types of regulation.
ER -
BOUCHAL, Pavel, Iva STRUHÁROVÁ, Eva BUDINSKÁ, Ondrej ŠEDO, Tereza VYHLÍDALOVÁ, Zbyněk ZDRÁHAL, Rob VAN SPANNING a Igor KUČERA. Unraveling an FNR based regulatory circuit in Paracoccus denitrificans using a proteomics-based approach. \textit{Biochimica et Biophysica Acta - Proteins and Proteomics}. Amsterdam, The Netherlands: Elsevier, roč.~1804, č.~6, s.~1309-1316. ISSN~1570-9639. doi:10.1016/j.bbapap.2010.01.016. 2010.
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