Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana

KLUMPLER, Tomáš, Blanka PEKÁROVÁ, Jaromír MAREK, Pavla BORKOVCOVÁ, Lubomír JANDA and Jan HEJÁTKO. Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana. In Kolokvium - Struktura 2009. 2009. ISSN 1211-5894.

Basic information

• Original name: Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
• Authors: KLUMPLER, Tomáš (203 Czech Republic, guarantor), Blanka PEKÁROVÁ (203 Czech Republic), Jaromír MAREK (203 Czech Republic), Pavla BORKOVCOVÁ (203 Czech Republic), Lubomír JANDA (203 Czech Republic) and Jan HEJÁTKO (203 Czech Republic).
• Edition: Kolokvium - Struktura 2009, 2009.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/09:00039824
• Organization unit: Faculty of Science
• ISSN: 1211-5894
• Keywords in English: histidinkinase CKI1 Arabidopsis cytokinin signaling crystal structure

Abstract

The crystal structure of the receiver domain (RD) of the histidine kinase CYTOKININ-INDEPENDENT1 (CKI1) from Arabidopsis thaliana has been determined at a resolution of 2.0 Angstroem. Crystals of the recombinant RD of CKI1 have been obtained. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4 Angstroem.The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 Angstroem, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. Initial R value of 0.54, which decreased to R = 0.413 and Rfree = 0.426 after 30 cycles of REFMAC refinement. The three-dimensional structure of A. thaliana CKI1RD shows the conformational conservation of receiver proteins, such as CheY, CheB, ETRRD. CKI1RD is a single domain protein folded in a Rossmann manner with a central beta-sheet formed from five beta-strands and surrounded by sides by two and three alpha-helices.

Links

• LC06034, research and development project: Name: Regulace morfogeneze rostlinných buněk a orgánů

Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations