Detailed Information on Publication Record
2009
Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
KLUMPLER, Tomáš, Blanka PEKÁROVÁ, Jaromír MAREK, Pavla BORKOVCOVÁ, Lubomír JANDA et. al.Basic information
Original name
Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana
Authors
KLUMPLER, Tomáš (203 Czech Republic, guarantor), Blanka PEKÁROVÁ (203 Czech Republic), Jaromír MAREK (203 Czech Republic), Pavla BORKOVCOVÁ (203 Czech Republic), Lubomír JANDA (203 Czech Republic) and Jan HEJÁTKO (203 Czech Republic)
Edition
Kolokvium - Struktura 2009, 2009
Other information
Language
English
Type of outcome
Konferenční abstrakt
Field of Study
Genetics and molecular biology
Country of publisher
Czech Republic
Confidentiality degree
není předmětem státního či obchodního tajemství
RIV identification code
RIV/00216224:14310/09:00039824
Organization unit
Faculty of Science
ISSN
Keywords in English
histidinkinase CKI1 Arabidopsis cytokinin signaling crystal structure
Změněno: 31/3/2010 10:51, Mgr. Tomáš Klumpler, Ph.D.
Abstract
V originále
The crystal structure of the receiver domain (RD) of the histidine kinase CYTOKININ-INDEPENDENT1 (CKI1) from Arabidopsis thaliana has been determined at a resolution of 2.0 Angstroem. Crystals of the recombinant RD of CKI1 have been obtained. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4 Angstroem.The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 Angstroem, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. Initial R value of 0.54, which decreased to R = 0.413 and Rfree = 0.426 after 30 cycles of REFMAC refinement. The three-dimensional structure of A. thaliana CKI1RD shows the conformational conservation of receiver proteins, such as CheY, CheB, ETRRD. CKI1RD is a single domain protein folded in a Rossmann manner with a central beta-sheet formed from five beta-strands and surrounded by sides by two and three alpha-helices.
Links
LC06034, research and development project |
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MSM0021622415, plan (intention) |
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