a 2009

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana

KLUMPLER, Tomáš, Blanka PEKÁROVÁ, Jaromír MAREK, Pavla BORKOVCOVÁ, Lubomír JANDA et. al.

Basic information

Original name

Crystal structure of the receiver domain of the histidine kinase CKI1 from Arabidopsis thaliana

Authors

KLUMPLER, Tomáš (203 Czech Republic, guarantor), Blanka PEKÁROVÁ (203 Czech Republic), Jaromír MAREK (203 Czech Republic), Pavla BORKOVCOVÁ (203 Czech Republic), Lubomír JANDA (203 Czech Republic) and Jan HEJÁTKO (203 Czech Republic)

Edition

Kolokvium - Struktura 2009, 2009

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/09:00039824

Organization unit

Faculty of Science

ISSN

Keywords in English

histidinkinase CKI1 Arabidopsis cytokinin signaling crystal structure
Změněno: 31/3/2010 10:51, Mgr. Tomáš Klumpler, Ph.D.

Abstract

V originále

The crystal structure of the receiver domain (RD) of the histidine kinase CYTOKININ-INDEPENDENT1 (CKI1) from Arabidopsis thaliana has been determined at a resolution of 2.0 Angstroem. Crystals of the recombinant RD of CKI1 have been obtained. The crystals diffracted at beamline BW7B of the DORIS-III storage ring to approx. 2.4 Angstroem.The crystals belong to space group C2221 with unit-cell parameters a=54.46, b=99.82, c=79.94 Angstroem, the asymmetric unit contains one molecule of the protein. The structure of CKI1RD had been solved by a molecular-replacement. Initial R value of 0.54, which decreased to R = 0.413 and Rfree = 0.426 after 30 cycles of REFMAC refinement. The three-dimensional structure of A. thaliana CKI1RD shows the conformational conservation of receiver proteins, such as CheY, CheB, ETRRD. CKI1RD is a single domain protein folded in a Rossmann manner with a central beta-sheet formed from five beta-strands and surrounded by sides by two and three alpha-helices.

Links

LC06034, research and development project
Name: Regulace morfogeneze rostlinných buněk a orgánů
Investor: Ministry of Education, Youth and Sports of the CR, Regulation of morphogenesis of plant cells and organs
MSM0021622415, plan (intention)
Name: Molekulární podstata buněčných a tkáňových regulací
Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations