Validated method for bioactive lignans in Schisandra chinensis
in vitro cultures using a solid phase extraction and a
monolithic column application

J 2010

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

BŘEZINOVÁ, Lenka; Helena VLAŠÍNOVÁ; Ladislav HAVEL; Otakar HUMPA; Jiří SLANINA et. al.

Základní údaje

Originální název

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Název česky

Validovaná metoda pro bioaktivní lignany ve Schisandra chinensis založená na extrakci pomocí pevné fáze a použití monolitické kolony

Autoři

BŘEZINOVÁ, Lenka (203 Česká republika, domácí); Helena VLAŠÍNOVÁ (203 Česká republika); Ladislav HAVEL (203 Česká republika); Otakar HUMPA (203 Česká republika, domácí) a Jiří SLANINA (203 Česká republika, garant, domácí)

Vydání

BIOMEDICAL CHROMATOGRAPHY, CHICHESTER, England, JOHN WILEY & SONS LTD, 2010, 0269-3879

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.545

Kód RIV

RIV/00216224:14110/10:00048590

Organizační jednotka

Lékařská fakulta

UT WoS

000281424600007

Klíčová slova česky

Schisandra chinensis; HPLC; monolithic column; dibenzocyclooctadiene lignans; plant cell culture

Klíčová slova anglicky

Schisandra chinensis; HPLC; monolithic column; dibenzocyclooctadiene lignans; plant cell culture

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 1. 2011 15:56, Mgr. Jiří Slanina, Ph.D.

Anotace

ORIG CZ

V originále

A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The base line separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 minutes), which was made possible by the relatively high flow rate of the mobile phase (2 ml/min). The lower limit of quantitation was 0.1 mg/l for schizandrin and gomisin A, 0.3 mg/l for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/l for wuweizisu C.

Česky

Jednoduchá a rychlá metoda pro stanovení 6 lignanů v buněčných kulturách Schisandra chinensis byla vyvinuta a validována

Návaznosti

GA522/07/0995, projekt VaV

Název: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis

Investor: Grantová agentura ČR, Regulace biosyntézy sekundárních metabolitů v buněčné kultuře Schisandra chinensis

Citovat

BŘEZINOVÁ, Lenka; Helena VLAŠÍNOVÁ; Ladislav HAVEL; Otakar HUMPA a Jiří SLANINA. Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application. BIOMEDICAL CHROMATOGRAPHY. CHICHESTER, England: JOHN WILEY & SONS LTD, 2010, roč. 24, č. 9, s. 954-960. ISSN 0269-3879.

@article{878530,
   author = {Březinová, Lenka and Vlašínová, Helena and Havel, Ladislav and Humpa, Otakar and Slanina, Jiří},
   article_location = {CHICHESTER, England},
   article_number = {9},
   keywords = {Schisandra chinensis; HPLC; monolithic column; dibenzocyclooctadiene lignans; plant cell culture},
   language = {eng},
   issn = {0269-3879},
   journal = {BIOMEDICAL CHROMATOGRAPHY},
   title = {Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application},
   volume = {24},
   year = {2010}
}

TY  - JOUR
ID  - 878530
AU  - Březinová, Lenka - Vlašínová, Helena - Havel, Ladislav - Humpa, Otakar - Slanina, Jiří
PY  - 2010
TI  - Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application
JF  - BIOMEDICAL CHROMATOGRAPHY
VL  - 24
IS  - 9
SP  - 954-960
EP  - 954-960
PB  - JOHN WILEY & SONS LTD
SN  - 02693879
KW  - Schisandra chinensis
KW  - HPLC
KW  - monolithic column
KW  - dibenzocyclooctadiene lignans
KW  - plant cell culture
N2  - A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The base line separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 minutes), which was made possible by the relatively high flow rate of the mobile phase (2 ml/min). The lower limit of quantitation was 0.1 mg/l for schizandrin and gomisin A, 0.3 mg/l for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/l for wuweizisu C.
ER  -

BŘEZINOVÁ, Lenka; Helena VLAŠÍNOVÁ; Ladislav HAVEL; Otakar HUMPA a Jiří SLANINA. Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application. \textit{BIOMEDICAL CHROMATOGRAPHY}. CHICHESTER, England: JOHN WILEY \&{} SONS LTD, 2010, roč.~24, č.~9, s.~954-960. ISSN~0269-3879.

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