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@article{878530,
author = {Březinová, Lenka and Vlašínová, Helena and Havel, Ladislav and Humpa, Otakar and Slanina, Jiří},
article_location = {CHICHESTER, England},
article_number = {9},
keywords = {Schisandra chinensis; HPLC; monolithic column; dibenzocyclooctadiene lignans; plant cell culture},
language = {eng},
issn = {0269-3879},
journal = {BIOMEDICAL CHROMATOGRAPHY},
title = {Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application},
volume = {24},
year = {2010}
}
TY - JOUR
ID - 878530
AU - Březinová, Lenka - Vlašínová, Helena - Havel, Ladislav - Humpa, Otakar - Slanina, Jiří
PY - 2010
TI - Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application
JF - BIOMEDICAL CHROMATOGRAPHY
VL - 24
IS - 9
SP - 954-960
EP - 954-960
PB - JOHN WILEY & SONS LTD
SN - 02693879
KW - Schisandra chinensis
KW - HPLC
KW - monolithic column
KW - dibenzocyclooctadiene lignans
KW - plant cell culture
N2 - A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The base line separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 minutes), which was made possible by the relatively high flow rate of the mobile phase (2 ml/min). The lower limit of quantitation was 0.1 mg/l for schizandrin and gomisin A, 0.3 mg/l for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/l for wuweizisu C.
ER -
BŘEZINOVÁ, Lenka, Helena VLAŠÍNOVÁ, Ladislav HAVEL, Otakar HUMPA and Jiří SLANINA. Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application. \textit{BIOMEDICAL CHROMATOGRAPHY}. CHICHESTER, England: JOHN WILEY \&{} SONS LTD, 2010, vol.~24, No~9, p.~954-960. ISSN~0269-3879.
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