Effect of quaternary benzo[c]phenanthridine alkaloids
sanguilutine and chelilutine on normal and cancer cells

J 2010

Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ, Iva SLANINOVÁ et. al.

Basic information

Original name

Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells

Name in Czech

Vliv kvarterních benzo [c] fenanthridinových alkaloidů sanguilutinu a chelilutinu na normální a nádorové buňky

Authors

SLUNSKÁ, Zdenka (203 Czech Republic), Eva GELNAROVÁ (203 Czech Republic), Jindřiška HAMMEROVÁ (203 Czech Republic, belonging to the institution), Eva TÁBORSKÁ (203 Czech Republic, belonging to the institution) and Iva SLANINOVÁ (203 Czech Republic, guarantor, belonging to the institution)

Edition

Toxicology in Vitro, Amsterodam, Elsevier Ltd. 2010, 0887-2333

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

Genetics and molecular biology

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 2.546

RIV identification code

RIV/00216224:14110/10:00043675

Organization unit

Faculty of Medicine

UT WoS

000276668500001

Keywords in English

benzo(c)phenanthridine alkaloids; sanguilutine; chelilutine; cytotoxicity; oxidative stress; antioxidant

Změněno: 14/1/2011 09:51, prof. MUDr. Iva Slaninová, Ph.D.

Abstract

V originále

Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid. All tested alkaloids, at concentrations of 0.1 and 1 ug/ml, did not increase ROS production. However, pre-incubation with all alkaloids, at all tested concentrations, with the exception of sanguilutine and chelerythrine at 1 ug/ml, decreased oxidative stress caused by H2O2. These findings indicate high anti-proliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction; to the contrary, both alkaloids showed anti-oxidative effects.

Links

GA525/08/0819, research and development project

Name: Rostlinné zdroje minoritních benzofenanthridinových alkaloidů a studium interakcí těchto alkaloidů s DNA

Investor: Czech Science Foundation, Plant sources of minor benzofenantridine alkaloids and study of heir interaction with DNA

LC06077, research and development project

Name: Centrum chemické genetiky

Investor: Ministry of Education, Youth and Sports of the CR

MSM0021622415, plan (intention)

Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

Citovat

SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ and Iva SLANINOVÁ. Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells. Toxicology in Vitro. Amsterodam: Elsevier Ltd., 2010, vol. 24, No 3, p. 697-706. ISSN 0887-2333.

@article{878739,
   author = {Slunská, Zdenka and Gelnarová, Eva and Hammerová, Jindřiška and Táborská, Eva and Slaninová, Iva},
   article_location = {Amsterodam},
   article_number = {3},
   keywords = {benzo(c)phenanthridine alkaloids; sanguilutine; chelilutine; cytotoxicity; oxidative stress; antioxidant},
   language = {eng},
   issn = {0887-2333},
   journal = {Toxicology in Vitro},
   title = {Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells},
   url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCP-4Y9SVS3-1&_user=835458&_coverDate=04%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=fb5acce5418506b30b592a4},
   volume = {24},
   year = {2010}
}

TY  - JOUR
ID  - 878739
AU  - Slunská, Zdenka - Gelnarová, Eva - Hammerová, Jindřiška - Táborská, Eva - Slaninová, Iva
PY  - 2010
TI  - Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells
JF  - Toxicology in Vitro
VL  - 24
IS  - 3
SP  - 697-706
EP  - 697-706
PB  - Elsevier Ltd.
SN  - 08872333
KW  - benzo(c)phenanthridine alkaloids
KW  - sanguilutine
KW  - chelilutine
KW  - cytotoxicity
KW  - oxidative stress
KW  - antioxidant
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCP-4Y9SVS3-1&_user=835458&_coverDate=04%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=fb5acce5418506b30b592a4
N2  - Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid. All tested alkaloids, at concentrations of 0.1 and 1 ug/ml, did not increase ROS production. However, pre-incubation with all alkaloids, at all tested concentrations, with the exception of sanguilutine and chelerythrine at 1 ug/ml, decreased oxidative stress caused by H2O2. These findings indicate high anti-proliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction; to the contrary, both alkaloids showed anti-oxidative effects.
ER  -

SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ and Iva SLANINOVÁ. Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells. \textit{Toxicology in Vitro}. Amsterodam: Elsevier Ltd., 2010, vol.~24, No~3, p.~697-706. ISSN~0887-2333.

Detailed Information on Publication Record