Other formats:
BibTeX
LaTeX
RIS
@article{878739,
author = {Slunská, Zdenka and Gelnarová, Eva and Hammerová, Jindřiška and Táborská, Eva and Slaninová, Iva},
article_location = {Amsterodam},
article_number = {3},
keywords = {benzo(c)phenanthridine alkaloids; sanguilutine; chelilutine; cytotoxicity; oxidative stress; antioxidant},
language = {eng},
issn = {0887-2333},
journal = {Toxicology in Vitro},
title = {Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells},
url = {http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCP-4Y9SVS3-1&_user=835458&_coverDate=04%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=fb5acce5418506b30b592a4},
volume = {24},
year = {2010}
}
TY - JOUR
ID - 878739
AU - Slunská, Zdenka - Gelnarová, Eva - Hammerová, Jindřiška - Táborská, Eva - Slaninová, Iva
PY - 2010
TI - Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells
JF - Toxicology in Vitro
VL - 24
IS - 3
SP - 697-706
EP - 697-706
PB - Elsevier Ltd.
SN - 08872333
KW - benzo(c)phenanthridine alkaloids
KW - sanguilutine
KW - chelilutine
KW - cytotoxicity
KW - oxidative stress
KW - antioxidant
UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6TCP-4Y9SVS3-1&_user=835458&_coverDate=04%2F30%2F2010&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000045159&_version=1&_urlVersion=0&_userid=835458&md5=fb5acce5418506b30b592a4
N2 - Sanguilutine and chelilutine, quaternary benzo[c]phenanthridine alkaloids, were studied for their anti-proliferative activities with regard to their ability to induce oxidative stress. We observed potent anti-proliferative activities for both alkaloids against three tumor (HeLa; HL-60; A-2780) and two normal (V-79; LEP) cell lines. Both alkaloids were efficient inductors of apoptosis. Statistical analysis revealed higher toxicity for sanguilutine compared to chelilutine and an unequal sensitivity with regard to individual cell lines but independent of the character of the cell line (i.e. tumor vs. normal). Dihydrofluorescein diacetate staining was used for measurement of intracellular ROS accumulation, not only after sanguilutine and chelilutine, but also after sanguinarine and chelerythrine treatment. In addition the antioxidative effect was studied. The effect of the alkaloids was compared with the effect of commonly used antioxidants such as trolox, caffeine acid, and chlorogenic acid. All tested alkaloids, at concentrations of 0.1 and 1 ug/ml, did not increase ROS production. However, pre-incubation with all alkaloids, at all tested concentrations, with the exception of sanguilutine and chelerythrine at 1 ug/ml, decreased oxidative stress caused by H2O2. These findings indicate high anti-proliferative and pro-apoptotic effects of sanguilutine and chelilutine that are not accompanied by oxidative stress induction; to the contrary, both alkaloids showed anti-oxidative effects.
ER -
SLUNSKÁ, Zdenka, Eva GELNAROVÁ, Jindřiška HAMMEROVÁ, Eva TÁBORSKÁ and Iva SLANINOVÁ. Effect of quaternary benzo[c]phenanthridine alkaloids sanguilutine and chelilutine on normal and cancer cells. \textit{Toxicology in Vitro}. Amsterodam: Elsevier Ltd., 2010, vol.~24, No~3, p.~697-706. ISSN~0887-2333.
|
