Drug efflux transporters, MRP1 and BCRP, affect the outcome of
hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma
cells

J 2009

Drug efflux transporters, MRP1 and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma cells

JENDZELOVSKY, Rastislav, Jaromir MIKES, Jan KOVAL, Karel SOUČEK, Jiřina PROCHÁZKOVÁ et. al.

Basic information

Original name

Drug efflux transporters, MRP1 and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma cells

Authors

JENDZELOVSKY, Rastislav (703 Slovakia), Jaromir MIKES (703 Slovakia), Jan KOVAL (703 Slovakia), Karel SOUČEK (203 Czech Republic), Jiřina PROCHÁZKOVÁ (203 Czech Republic), Martin KELLO (703 Slovakia), Veronika SACKOVA (703 Slovakia), Jiřina HOFMANOVÁ (203 Czech Republic), Alois KOZUBÍK (203 Czech Republic, guarantor) and Peter FEDOROČKO (703 Slovakia)

Edition

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2009, 1474-905X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30200 3.2 Clinical medicine

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.708

RIV identification code

RIV/00216224:14310/09:00039943

Organization unit

Faculty of Science

UT WoS

000272142300010

Keywords in English

MULTIDRUG-RESISTANCE; P-GLYCOPROTEIN; JOHNS-WORT; MXR ABCG2; METABOLISM; TOXICITY; INHIBITORS; INDUCTION; MODULATION; EXPRESSION

Abstract

V originále

Photodynamic therapy (PDT) is a flexible multi-target therapeutic approach. Mechanisms of anticancer drug elimination by tumour cells are mostly linked to the elevated expression and activity of P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1), breast cancer resistance protein (BCRP) and P450 monooxygenases. We report here for the first time increased activity of MRP1 and BCRP in HT-29 colon cancer cells treated with hypericin per se. On the contrary, pre-treatment with proadifen (SKF525A) affected the function of MRP1 and BCRP leading to increased hypericin content, which might indicate a possible link between proadifen and these ABC transporter proteins. Subsequent enhanced intracellular oxidative stress was accompanied by loss of mitochondrial membrane potential, activation of caspase-9 and -3, PARP cleavage and onset of apoptosis.

Citovat

JENDZELOVSKY, Rastislav, Jaromir MIKES, Jan KOVAL, Karel SOUČEK, Jiřina PROCHÁZKOVÁ, Martin KELLO, Veronika SACKOVA, Jiřina HOFMANOVÁ, Alois KOZUBÍK and Peter FEDOROČKO. Drug efflux transporters, MRP1 and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma cells. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES. 2009, vol. 8, No 12, 8 pp. ISSN 1474-905X.

@article{879431,
   author = {Jendzelovsky, Rastislav and Mikes, Jaromir and Koval, Jan and Souček, Karel and Procházková, Jiřina and Kello, Martin and Sackova, Veronika and Hofmanová, Jiřina and Kozubík, Alois and Fedoročko, Peter},
   article_number = {12},
   keywords = {MULTIDRUG-RESISTANCE; P-GLYCOPROTEIN; JOHNS-WORT; MXR ABCG2; METABOLISM; TOXICITY; INHIBITORS; INDUCTION; MODULATION; EXPRESSION},
   language = {eng},
   issn = {1474-905X},
   journal = {PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES},
   title = {Drug efflux transporters, MRP1 and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma cells},
   volume = {8},
   year = {2009}
}

TY  - JOUR
ID  - 879431
AU  - Jendzelovsky, Rastislav - Mikes, Jaromir - Koval, Jan - Souček, Karel - Procházková, Jiřina - Kello, Martin - Sackova, Veronika - Hofmanová, Jiřina - Kozubík, Alois - Fedoročko, Peter
PY  - 2009
TI  - Drug efflux transporters, MRP1 and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma cells
JF  - PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
VL  - 8
IS  - 12
SN  - 1474905X
KW  - MULTIDRUG-RESISTANCE
KW  - P-GLYCOPROTEIN
KW  - JOHNS-WORT
KW  - MXR ABCG2
KW  - METABOLISM
KW  - TOXICITY
KW  - INHIBITORS
KW  - INDUCTION
KW  - MODULATION
KW  - EXPRESSION
N2  - Photodynamic therapy (PDT) is a flexible multi-target therapeutic approach. Mechanisms of anticancer drug elimination by tumour cells are mostly linked to the elevated expression and activity of P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1), breast cancer resistance protein (BCRP) and P450 monooxygenases. We report here for the first time increased activity of MRP1 and BCRP in HT-29 colon cancer cells treated with hypericin per se. On the contrary, pre-treatment with proadifen (SKF525A) affected the function of MRP1 and BCRP leading to increased hypericin content, which might indicate a possible link between proadifen and these ABC transporter proteins. Subsequent enhanced intracellular oxidative stress was accompanied by loss of mitochondrial membrane potential, activation of caspase-9 and -3, PARP cleavage and onset of apoptosis.
ER  -

JENDZELOVSKY, Rastislav, Jaromir MIKES, Jan KOVAL, Karel SOUČEK, Jiřina PROCHÁZKOVÁ, Martin KELLO, Veronika SACKOVA, Jiřina HOFMANOVÁ, Alois KOZUBÍK and Peter FEDOROČKO. Drug efflux transporters, MRP1 and BCRP, affect the outcome of hypericin-mediated photodynamic therapy in HT-29 adenocarcinoma cells. \textit{PHOTOCHEMICAL \&{} PHOTOBIOLOGICAL SCIENCES}. 2009, vol.~8, No~12, 8 pp. ISSN~1474-905X.

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