Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances

KANCHEVA, Radmila, Martin HILL, Zdeněk NOVÁK, Jan CHRASTINA, Marta VELIKOVA, Lyudmila KANCHEVA, Ivo ŘÍHA and Luboslav STARKA. Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances. Journal of Steroid Biochemistry and Molecular Biology. England: Elsevier Science, 2010, vol. 119, 1-2, p. 35-44. ISSN 0960-0760. Available from: https://dx.doi.org/10.1016/j.jsbmb.2009.12.006.

Basic information

Original name

Peripheral neuroactive steroids may be as good as the steroids in the cerebrospinal fluid for the diagnostics of CNS disturbances

Name in Czech

Periferní neuroaktivní steroidy mohou být stejně přínosné jako steroidy v mozkomíšním moku pro diagnostiku onemocnění centrálního nervového systému

Authors

KANCHEVA, Radmila (203 Czech Republic, guarantor), Martin HILL (203 Czech Republic), Zdeněk NOVÁK (203 Czech Republic, belonging to the institution), Jan CHRASTINA (203 Czech Republic, belonging to the institution), Marta VELIKOVA (203 Czech Republic), Lyudmila KANCHEVA (203 Czech Republic), Ivo ŘÍHA (203 Czech Republic, belonging to the institution) and Luboslav STARKA (203 Czech Republic)

Edition

Journal of Steroid Biochemistry and Molecular Biology, England, Elsevier Science, 2010, 0960-0760

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Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

30000 3. Medical and Health Sciences

Country of publisher

United Kingdom of Great Britain and Northern Ireland

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 2.886

RIV identification code

RIV/00216224:14110/10:00051346

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1016/j.jsbmb.2009.12.006

UT WoS

000276259500004

Keywords (in Czech)

Steroidy; Mozkomíšní mok (CSF); Plasma; Metabolome; GC-MS.

Keywords in English

Steroids; Cerebrospinal fluid (CSF); Plasma; Metabolome; GC-MS

Tags

International impact

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Abstract

V originále

To compare the predictivity of the neuroactive steroids in the cerebrospinal fluid and peripheral blood for the diagnostics of CNS disturbances, eighteen unconjugated steroids were quantified in the cerebrospinal fluid (CSF) from the 3rd ventricle and 18 unconjugated steroids and 7 steroid polar conjugates were measured in the serum using GC-MS and RIA. Eight postmenopausal women (56-78 years of age) and 7 men (22-88 years of age) with hydrocephalus were enrolled in the study. The sensitivity of the method ranged from low femtogram to low picogram levels depending on the steroid fragmentation pattern. Using multivariate regression, a model for simultaneous prediction of the CSF steroids from the serum steroids was completed. Then, the penetrability of the individual steroids across the blood-brain-barrier was evaluated and the sources of various brain steroids were estimated. Our data show that a part of the steroids may be synthesized de novo in the CNS. However, substantial part of the steroid metabolites may be synthesized in the CNS from the steroid precursors or directly transported from the periphery. The CNS in situ synthesis and transport from periphery might be complementary in some cases, i.e. brain synthesis might provide minimum level of steroids, which are indispensable for the CNS functions.

In Czech

Získaná data ukazují, že část steroidů může být syntetizována de novo v centrálním nervovém systému. Ovšem podstatná část steroidních metabolitů může být v CNS syntezována ze steroidních prekurzorů nebo přímo transportována z periferie. Syntéza v CN a transport z periferie se mohou v určitých případech doplňovat, například mozková synteza by mohla poskytnout minimální hladiny steroidů, která je nutná pro funkci mozku.