Comparative evaluation of automated ribotyping and RAPD-PCR for
typing of Lactobacillus spp. occurring in dental caries

J 2010

Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries

ŠVEC, Pavel, Martina KUKLETOVÁ and Ivo SEDLÁČEK

Basic information

Original name

Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries

Authors

ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution)

Edition

Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Springer, 2010, 0003-6072

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 1.673

RIV identification code

RIV/00216224:14310/10:00043797

Organization unit

Faculty of Science

UT WoS

000276902800009

Keywords in English

Dental caries; Lactobacillus; Randomly amplified polymorphic DNA (RAPD-PCR); RiboPrinter; Ribotyping

Abstract

V originále

A group of 67 Lactobacillus spp. strains containing Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (Švec et al., Folia Microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the RiboPrinter microbial characterization system and by randomly amplified polymorphic DNA fingerprinting (RAPD-PCR) with M13 primer to evaluate these techniques for characterization of lactobacilli associated with dental caries. Ribotyping revealed 55 riboprints among the analysed group. The automatic identification process performed by the RiboPrinter system identified 18 strains to the species level, however cluster analysis divided obtained ribotype patterns into individual clusters mostly corresponding to the species assignment of particular strains. RAPD-PCR fingerprints revealed by the individual Lactobacillus spp. showed higher variability than the ribotype patterns and the fingerprint profiles generated by the analysed species were distributed among one to four clusters. In conclusion, ribotyping is shown to be more convenient for the identification purposes while RAPD-PCR fingerprinting results indicate this method is a better tool for typing of Lactobacillus spp. strains occurring in dental caries.

Links

GA310/09/0657, research and development project

Name: Molekulární taxonomie grampozitivních bakterií izolovaných ze zubního kazu v dočasné dentici

Investor: Czech Science Foundation, Molecular taxonomy of Gram-positive bacteria isolated from early childhood caries

MSM0021622416, plan (intention)

Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase

Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time

1M0528, research and development project

Name: Stomatologické výzkumné centrum

Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre

Citovat

ŠVEC, Pavel, Martina KUKLETOVÁ and Ivo SEDLÁČEK. Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Springer, 2010, vol. 98, No 1, p. 85-92. ISSN 0003-6072.

@article{881114,
   author = {Švec, Pavel and Kukletová, Martina and Sedláček, Ivo},
   article_number = {1},
   keywords = {Dental caries; Lactobacillus; Randomly amplified polymorphic DNA (RAPD-PCR); RiboPrinter; Ribotyping},
   language = {eng},
   issn = {0003-6072},
   journal = {Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology},
   title = {Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries},
   url = {http://www.springerlink.com/content/lv85174706rg56p1/?p=31e5ef171e0e42678745e028ba8d3e31&pi=8},
   volume = {98},
   year = {2010}
}

TY  - JOUR
ID  - 881114
AU  - Švec, Pavel - Kukletová, Martina - Sedláček, Ivo
PY  - 2010
TI  - Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries
JF  - Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology
VL  - 98
IS  - 1
SP  - 85-92
EP  - 85-92
PB  - Springer
SN  - 00036072
KW  - Dental caries
KW  - Lactobacillus
KW  - Randomly amplified polymorphic DNA (RAPD-PCR)
KW  - RiboPrinter
KW  - Ribotyping
UR  - http://www.springerlink.com/content/lv85174706rg56p1/?p=31e5ef171e0e42678745e028ba8d3e31&pi=8
N2  - A group of 67 Lactobacillus spp. strains containing Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (Švec et al., Folia Microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the RiboPrinter microbial characterization system and by randomly amplified polymorphic DNA fingerprinting (RAPD-PCR) with M13 primer to evaluate these techniques for characterization of lactobacilli associated with dental caries. Ribotyping revealed 55 riboprints among the analysed group. The automatic identification process performed by the RiboPrinter system identified 18 strains to the species level, however cluster analysis divided obtained ribotype patterns into individual clusters mostly corresponding to the species assignment of particular strains. RAPD-PCR fingerprints revealed by the individual Lactobacillus spp. showed higher variability than the ribotype patterns and the fingerprint profiles generated by the analysed species were distributed among one to four clusters. In conclusion, ribotyping is shown to be more convenient for the identification purposes while RAPD-PCR fingerprinting results indicate this method is a better tool for typing of Lactobacillus spp. strains occurring in dental caries.
ER  -

ŠVEC, Pavel, Martina KUKLETOVÁ and Ivo SEDLÁČEK. Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries. \textit{Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology}. Springer, 2010, vol.~98, No~1, p.~85-92. ISSN~0003-6072.

Detailed Information on Publication Record