ŠVEC, Pavel, Martina KUKLETOVÁ and Ivo SEDLÁČEK. Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries. Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology. Springer, 2010, vol. 98, No 1, p. 85-92. ISSN 0003-6072.
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Basic information
Original name Comparative evaluation of automated ribotyping and RAPD-PCR for typing of Lactobacillus spp. occurring in dental caries
Authors ŠVEC, Pavel (203 Czech Republic, guarantor, belonging to the institution), Martina KUKLETOVÁ (203 Czech Republic, belonging to the institution) and Ivo SEDLÁČEK (203 Czech Republic, belonging to the institution).
Edition Antonie van Leeuwenhoek International Journal of General and Molecular Microbiology, Springer, 2010, 0003-6072.
Other information
Original language English
Type of outcome Article in a journal
Field of Study 10600 1.6 Biological sciences
Country of publisher Netherlands
Confidentiality degree is not subject to a state or trade secret
WWW URL
Impact factor Impact factor: 1.673
RIV identification code RIV/00216224:14310/10:00043797
Organization unit Faculty of Science
UT WoS 000276902800009
Keywords in English Dental caries; Lactobacillus; Randomly amplified polymorphic DNA (RAPD-PCR); RiboPrinter; Ribotyping
Tags International impact, Reviewed
Changed by Changed by: doc. RNDr. Pavel Švec, Ph.D., učo 1098. Changed: 21/3/2011 07:30.
Abstract
A group of 67 Lactobacillus spp. strains containing Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previous study (Švec et al., Folia Microbiol 54:53-58, 2009) was characterized by automated ribotyping performed by the RiboPrinter microbial characterization system and by randomly amplified polymorphic DNA fingerprinting (RAPD-PCR) with M13 primer to evaluate these techniques for characterization of lactobacilli associated with dental caries. Ribotyping revealed 55 riboprints among the analysed group. The automatic identification process performed by the RiboPrinter system identified 18 strains to the species level, however cluster analysis divided obtained ribotype patterns into individual clusters mostly corresponding to the species assignment of particular strains. RAPD-PCR fingerprints revealed by the individual Lactobacillus spp. showed higher variability than the ribotype patterns and the fingerprint profiles generated by the analysed species were distributed among one to four clusters. In conclusion, ribotyping is shown to be more convenient for the identification purposes while RAPD-PCR fingerprinting results indicate this method is a better tool for typing of Lactobacillus spp. strains occurring in dental caries.
Links
GA310/09/0657, research and development projectName: Molekulární taxonomie grampozitivních bakterií izolovaných ze zubního kazu v dočasné dentici
Investor: Czech Science Foundation, Molecular taxonomy of Gram-positive bacteria isolated from early childhood caries
MSM0021622416, plan (intention)Name: Diverzita biotických společenstev a populací: kauzální analýza variability v prostoru a čase
Investor: Ministry of Education, Youth and Sports of the CR, Diversity of Biotic Communities and Populations: Causal Analysis of variation in space and time
1M0528, research and development projectName: Stomatologické výzkumné centrum
Investor: Ministry of Education, Youth and Sports of the CR, Dental research centre
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