c-Myb promotes motility and invasion of breast cancer cells

KNOPFOVÁ, Lucia, Filip TRČKA, Petr BENEŠ and Jan ŠMARDA. c-Myb promotes motility and invasion of breast cancer cells. In The 6th symposium and workshop on molecular pathology and histo(cyto)chemistry. 2010. ISBN 978-80-87327-07-4.

Basic information

• Original name: c-Myb promotes motility and invasion of breast cancer cells
• Name in Czech: Protein c-Myb zvyšuje motilitu a invazivitu buněk prsního karcinomu
• Authors: KNOPFOVÁ, Lucia (203 Czech Republic, belonging to the institution), Filip TRČKA (203 Czech Republic, belonging to the institution), Petr BENEŠ (203 Czech Republic, belonging to the institution) and Jan ŠMARDA (203 Czech Republic, guarantor, belonging to the institution).
• Edition: The 6th symposium and workshop on molecular pathology and histo(cyto)chemistry, 2010.

Other information

• Original language: English
• Type of outcome: Conference abstract
• Field of Study: Genetics and molecular biology
• Country of publisher: Czech Republic
• Confidentiality degree: is not subject to a state or trade secret
• RIV identification code: RIV/00216224:14310/10:00040519
• Organization unit: Faculty of Science
• ISBN: 978-80-87327-07-4
• Keywords (in Czech): c-Myb; invazivita; migrace; prsní karcinom
• Keywords in English: c-Myb; invasion; migration; breast carcinoma
• Tags: c-Myb, invazivita

Abstract

The aim of this study was to assess the role of the c-Myb protein in control of invasion of breast cancer cells. MDA-MB-231 breast cancer cells were transfected with the c-Myb coding cDNA to prepare MDA-MB-231MYBup derivatives. The effects of c-Myb overexpression on migration and invasion capacity of these cells were assessed. MDA-MB-231MYBup cells were significantly more active in both motility and invasion than controls. In order to reveal dynamics of these processes, we performed real-time analysis of cell migration and invasion using the xCELLigence RT-CA system. This analysis clearly confirmed increased invasive capacity of MDA-MB-231MYBup cells compared to controls. To address the mechanism of c-Myb-enhanced breast cancer cell invasion, we examined the role of c-Myb in control of expression and activity of some of the proteases involved in degradation of extracellular matrix. We found that c-Myb increased production of cathepsin D and matrix metalloproteinases in MDA-MB-231MYBup cells.

Abstract (in Czech)

c-Myb zvyšuje motilitu a invazivitu buněk prsního karcinomu MDA-MB-231 cells. Zvýšená invazivita může být důsledkem deregulace exprese/aktivity proteáz degradujících ECM matrix metalloproteinázy 9 and cathepsinu D. Naše výsledky naznačují novou funkci proteinu c-Myb v regulaci invazivity nádorů and metastatické progrese.

Links

• GA301/09/1115, research and development project: Name: Úloha proteinů c-Myb a Cox-2 při tvorbě střevních nádorů

Investor: Czech Science Foundation, The role of c-Myb and Cox-2 proteins in colon carcinogenesis

• IAA501630801, research and development project: Name: Mechanismus účinku prokatepsinu D na buňky rakoviny prsu

Investor: Academy of Sciences of the Czech Republic, Mechanism of procathepsin D effect on breast cancer cells

• MSM0021622415, plan (intention): Name: Molekulární podstata buněčných a tkáňových regulací

Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations

• MUNI/C/0099/2009, interní kód MU: Name: Význam proteinu c-Myb pro invazivitu buněk střevních a prsních karcinomů

Investor: Masaryk University, Category C - centralized resources + specific research