c-Myb promotes motility and invasion of breast cancer cells

KNOPFOVÁ, Lucia, Filip TRČKA, Petr BENEŠ and Jan ŠMARDA. c-Myb promotes motility and invasion of breast cancer cells. In The 6th symposium and workshop on molecular pathology and histo(cyto)chemistry. 2010. ISBN 978-80-87327-07-4.

Basic information

Original name

c-Myb promotes motility and invasion of breast cancer cells

Name in Czech

Protein c-Myb zvyšuje motilitu a invazivitu buněk prsního karcinomu

Authors

KNOPFOVÁ, Lucia (203 Czech Republic, belonging to the institution), Filip TRČKA (203 Czech Republic, belonging to the institution), Petr BENEŠ (203 Czech Republic, belonging to the institution) and Jan ŠMARDA (203 Czech Republic, guarantor, belonging to the institution)

Edition

The 6th symposium and workshop on molecular pathology and histo(cyto)chemistry, 2010

---

Other information

Language

English

Type of outcome

Konferenční abstrakt

Field of Study

Genetics and molecular biology

Country of publisher

Czech Republic

Confidentiality degree

není předmětem státního či obchodního tajemství

RIV identification code

RIV/00216224:14310/10:00040519

Organization unit

Faculty of Science

ISBN

978-80-87327-07-4

Keywords (in Czech)

c-Myb; invazivita; migrace; prsní karcinom

Keywords in English

c-Myb; invasion; migration; breast carcinoma

Tags

c-Myb, invazivita

---

Abstract

V originále

The aim of this study was to assess the role of the c-Myb protein in control of invasion of breast cancer cells. MDA-MB-231 breast cancer cells were transfected with the c-Myb coding cDNA to prepare MDA-MB-231MYBup derivatives. The effects of c-Myb overexpression on migration and invasion capacity of these cells were assessed. MDA-MB-231MYBup cells were significantly more active in both motility and invasion than controls. In order to reveal dynamics of these processes, we performed real-time analysis of cell migration and invasion using the xCELLigence RT-CA system. This analysis clearly confirmed increased invasive capacity of MDA-MB-231MYBup cells compared to controls. To address the mechanism of c-Myb-enhanced breast cancer cell invasion, we examined the role of c-Myb in control of expression and activity of some of the proteases involved in degradation of extracellular matrix. We found that c-Myb increased production of cathepsin D and matrix metalloproteinases in MDA-MB-231MYBup cells.

In Czech

c-Myb zvyšuje motilitu a invazivitu buněk prsního karcinomu MDA-MB-231 cells. Zvýšená invazivita může být důsledkem deregulace exprese/aktivity proteáz degradujících ECM matrix metalloproteinázy 9 and cathepsinu D. Naše výsledky naznačují novou funkci proteinu c-Myb v regulaci invazivity nádorů and metastatické progrese.

---

Links

GA301/09/1115, research and development project	Name: Úloha proteinů c-Myb a Cox-2 při tvorbě střevních nádorů Investor: Czech Science Foundation, The role of c-Myb and Cox-2 proteins in colon carcinogenesis
IAA501630801, research and development project	Name: Mechanismus účinku prokatepsinu D na buňky rakoviny prsu Investor: Academy of Sciences of the Czech Republic, Mechanism of procathepsin D effect on breast cancer cells
MSM0021622415, plan (intention)	Name: Molekulární podstata buněčných a tkáňových regulací Investor: Ministry of Education, Youth and Sports of the CR, Molecular basis of cell and tissue regulations
MUNI/C/0099/2009, interní kód MU	Name: Význam proteinu c-Myb pro invazivitu buněk střevních a prsních karcinomů Investor: Masaryk University, Category C - centralized resources + specific research