| MOTÁČKOVÁ, Veronika, Hana ŠANDEROVÁ, Lukáš ŽÍDEK, Jiří NOVÁČEK, Petr PADRTA, Alžběta ŠVENKOVÁ, Jana KORELUSOVÁ, Jiří JONÁK, Libor KRÁSNÝ and Vladimír SKLENÁŘ. Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs. Proteins: Structure, Function, and Bioinformatics. USA: John Wiley and Sons, 2010, vol. 78, No 7, p. 1807-1810. ISSN 0887-3585. |
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@article{883410,
author = {Motáčková, Veronika and Šanderová, Hana and Žídek, Lukáš and Nováček, Jiří and Padrta, Petr and Švenková, Alžběta and Korelusová, Jana and Jonák, Jiří and Krásný, Libor and Sklenář, Vladimír},
article_location = {USA},
article_number = {7},
keywords = {RNA polymerase; delta subunit; gram positive bacteria; nuclear magnetic resonance; structural homologues},
language = {eng},
issn = {0887-3585},
journal = {Proteins: Structure, Function, and Bioinformatics},
title = {Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs},
volume = {78},
year = {2010}
}
TY - JOUR
ID - 883410
AU - Motáčková, Veronika - Šanderová, Hana - Žídek, Lukáš - Nováček, Jiří - Padrta, Petr - Švenková, Alžběta - Korelusová, Jana - Jonák, Jiří - Krásný, Libor - Sklenář, Vladimír
PY - 2010
TI - Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs
JF - Proteins: Structure, Function, and Bioinformatics
VL - 78
IS - 7
SP - 1807-1810
EP - 1807-1810
PB - John Wiley and Sons
SN - 08873585
KW - RNA polymerase
KW - delta subunit
KW - gram positive bacteria
KW - nuclear magnetic resonance
KW - structural homologues
N2 - RNA polymerase is an essential multisubunit enzyme responsible for transcription of genetic information from DNA into RNA. The RNA polymerase from Bacillus subtilis differs from its analogue from gram-negative bacteria in a presence of two additional subunits, omega1 and delta. Their role in the transcription machinery is still not clear. In this study, we focused on the N-terminal part of delta subunit to reveal its structure. The sample was prepared using a standard protocol of overexpression in the E.coli system to produce a 15N,13C-uniformly labeled sample. A standard set of spectra was measured on a 600MHz spectrometer. The distance restrains were extracted and assigned from NOESY spectra. The additional RDC restraints and anisotropic contributions to the 13C chemical shifts were used in the final refinement. The quality of the calculated structures were checked. The determined structure was identified based on structure homology with some proteins from the Forkhead DNA-binding domain SCOP family.
ER -
MOTÁČKOVÁ, Veronika, Hana ŠANDEROVÁ, Lukáš ŽÍDEK, Jiří NOVÁČEK, Petr PADRTA, Alžběta ŠVENKOVÁ, Jana KORELUSOVÁ, Jiří JONÁK, Libor KRÁSNÝ and Vladimír SKLENÁŘ. Solution structure of the N-terminal domain of Bacillus subtilis delta subunit of RNA polymerase and its classification based on structural homologs. \textit{Proteins: Structure, Function, and Bioinformatics}. USA: John Wiley and Sons, 2010, vol.~78, No~7, p.~1807-1810. ISSN~0887-3585.
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