FOLTYNOVÁ, Pavla, Jarmila NAVRÁTILOVÁ, Ondřej PEŠ, Tomáš VACULOVIČ, Viktor KANICKÝ, Jan ŠMARDA and Jan PREISLER. Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of copper in myeloid leukemia cells. Journal of Analytical Atomic Spectrometry. ENGLAND: ROYAL SOC CHEMISTRY, 2010, vol. 25, No 5, p. 662-668. ISSN 0267-9477. |
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@article{883989, author = {Foltynová, Pavla and Navrátilová, Jarmila and Peš, Ondřej and Vaculovič, Tomáš and Kanický, Viktor and Šmarda, Jan and Preisler, Jan}, article_location = {ENGLAND}, article_number = {5}, keywords = {ATOMIC-ABSORPTION-SPECTROMETRY; LA-ICP-MS; CAPILLARY-ELECTROPHORESIS; CALIBRATION STRATEGY; GEL-ELECTROPHORESIS; PROTEASOME ACTIVITY; BIOLOGICAL SAMPLES; TRACE-METALS; ABLATION; DISULFIRAM}, language = {eng}, issn = {0267-9477}, journal = {Journal of Analytical Atomic Spectrometry}, title = {Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of copper in myeloid leukemia cells}, volume = {25}, year = {2010} }
TY - JOUR ID - 883989 AU - Foltynová, Pavla - Navrátilová, Jarmila - Peš, Ondřej - Vaculovič, Tomáš - Kanický, Viktor - Šmarda, Jan - Preisler, Jan PY - 2010 TI - Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of copper in myeloid leukemia cells JF - Journal of Analytical Atomic Spectrometry VL - 25 IS - 5 SP - 662-668 EP - 662-668 PB - ROYAL SOC CHEMISTRY SN - 02679477 KW - ATOMIC-ABSORPTION-SPECTROMETRY KW - LA-ICP-MS KW - CAPILLARY-ELECTROPHORESIS KW - CALIBRATION STRATEGY KW - GEL-ELECTROPHORESIS KW - PROTEASOME ACTIVITY KW - BIOLOGICAL SAMPLES KW - TRACE-METALS KW - ABLATION KW - DISULFIRAM N2 - This work presents a detailed characterization of substrate-assisted laser desorption inductively coupled plasma mass spectrometry (SALD ICP-MS), a new approach in the determination of trace elements in submicrolitre volumes. SALD ICP-MS allows quantitative analysis of metals in liquid samples with defined volumes deposited onto an absorbing substrate due to complete sample desorption at relatively low laser power density. Analysis of model samples of Cu(II) showed a constant signal over a wide range of laser power density, an independence of signal on desorption modes and a wide linear dynamic range. SALD ICP-MS with its limit of detection for copper below 26 fg in submicrolitre samples was successfully applied to investigate cytotoxic effects of disulfiram in human myeloid leukemia cells U937 depending on intracellular levels of copper. ER -
FOLTYNOVÁ, Pavla, Jarmila NAVRÁTILOVÁ, Ondřej PEŠ, Tomáš VACULOVIČ, Viktor KANICKÝ, Jan ŠMARDA and Jan PREISLER. Substrate-assisted laser desorption inductively-coupled plasma mass spectrometry for determination of copper in myeloid leukemia cells. \textit{Journal of Analytical Atomic Spectrometry}. ENGLAND: ROYAL SOC CHEMISTRY, 2010, vol.~25, No~5, p.~662-668. ISSN~0267-9477.
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