| KAVAN, Daniel, Monika KUBÍČKOVÁ, Jan BILÝ, Ondřej VANĚK, Kateřina HOFBAUEROVÁ, Mrázek HYNEK, Rozbeský DANIEL, Bojarová PAVLA, Vladimír KŘEN, Lukáš ŽÍDEK, Vladimír SKLENÁŘ and Karel BEZOUŠKA. Cooperativity between subunits is essential for high affinity binding of N-acetylhexosamines to dimeric soluble and dimeric cellular forms of human CD69. Biochemistry. Washington, USA: American Chemical Society, 2010, vol. 49, No 19, p. 4060-4067. ISSN 0006-2960. |
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@article{885673,
author = {Kavan, Daniel and Kubíčková, Monika and Bilý, Jan and Vaněk, Ondřej and Hofbauerová, Kateřina and Hynek, Mrázek and Daniel, Rozbeský and Pavla, Bojarová and Křen, Vladimír and Žídek, Lukáš and Sklenář, Vladimír and Bezouška, Karel},
article_location = {Washington, USA},
article_number = {19},
keywords = {NMR CD69 lectin-type proteins NK cell glycoproteins},
language = {eng},
issn = {0006-2960},
journal = {Biochemistry},
title = {Cooperativity between subunits is essential for high affinity binding of N-acetylhexosamines to dimeric soluble and dimeric cellular forms of human CD69},
volume = {49},
year = {2010}
}
TY - JOUR
ID - 885673
AU - Kavan, Daniel - Kubíčková, Monika - Bilý, Jan - Vaněk, Ondřej - Hofbauerová, Kateřina - Hynek, Mrázek - Daniel, Rozbeský - Pavla, Bojarová - Křen, Vladimír - Žídek, Lukáš - Sklenář, Vladimír - Bezouška, Karel
PY - 2010
TI - Cooperativity between subunits is essential for high affinity binding of N-acetylhexosamines to dimeric soluble and dimeric cellular forms of human CD69
JF - Biochemistry
VL - 49
IS - 19
SP - 4060-4067
EP - 4060-4067
PB - American Chemical Society
SN - 00062960
KW - NMR CD69 lectin-type proteins NK cell glycoproteins
N2 - Binding of GlcNAc to dimeric human CD69 was followed by equilibrium dialysis, fluorescence titration, and NMR. Clear cooperativity in high affinity binding to two subunits was observed (Hill coefficient 1.94), and binding of the ligand was connected with opening of dimer structure. However, monomeric CD69 obtained by mutating Q93 and R134 at dimer interface showed much lower affinity and no cooperativity (Hill coefficient 1.07). Perturbation of dimer interface resulted in severe impairment of the signaling ability of cellular CD69 cross-linked with antibodies or a bivalent high affinity N-acetylhexosamine-based ligand.
ER -
KAVAN, Daniel, Monika KUBÍČKOVÁ, Jan BILÝ, Ondřej VANĚK, Kateřina HOFBAUEROVÁ, Mrázek HYNEK, Rozbeský DANIEL, Bojarová PAVLA, Vladimír KŘEN, Lukáš ŽÍDEK, Vladimír SKLENÁŘ and Karel BEZOUŠKA. Cooperativity between subunits is essential for high affinity binding of N-acetylhexosamines to dimeric soluble and dimeric cellular forms of human CD69. \textit{Biochemistry}. Washington, USA: American Chemical Society, 2010, vol.~49, No~19, p.~4060-4067. ISSN~0006-2960.
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